Svensson A M, Borg L A, Eriksson U J
Department of Medical Cell Biology, University of Uppsala, Sweden.
Acta Endocrinol (Copenh). 1992 Sep;127(3):252-7. doi: 10.1530/acta.0.1270252.
To investigate whether malformations in the offspring of diabetic rats are related to a disturbed embryonic glucose metabolism, lactate production, lactate content and glucose oxidation in embryos of normal and manifestly diabetic rats were measured in vitro during the period of organogenesis. The embryonic production and content of lactate decreased by 90% between gestational days 10 and 11. On both gestational days the embryos of diabetic rats showed increased lactate production and content compared to the embryos of normal rats. The embryonic oxidation of glucose decreased slightly from gestational day 10 to day 11, and the embryos of diabetic rats exhibited a lower glucose oxidation rate than those of normal rats on both days. The lactate production and content in the embryos increased with increasing concentrations of glucose in the incubation medium, whereas the oxidation of glucose was unaffected by the glucose concentration. The results suggest that the embryonic metabolism of glucose is only marginally affected by changes in the availability of glucose. The lactate production rate was higher and the glucose oxidation rate lower in the embryos of diabetic than in those of normal rats, even when the values for these metabolic variables were normalized to the embryonic DNA content. Thus, a causative role for a disturbance of the glucose metabolism in diabetes-induced embryonic dysmorphogenesis could be envisaged.
为研究糖尿病大鼠后代的畸形是否与胚胎期葡萄糖代谢紊乱有关，在器官发生期，体外测量了正常大鼠和显性糖尿病大鼠胚胎中的乳酸生成、乳酸含量及葡萄糖氧化情况。在妊娠第10天到第11天期间，胚胎乳酸生成量和含量下降了90%。在这两个妊娠日，与正常大鼠胚胎相比，糖尿病大鼠胚胎的乳酸生成量和含量均增加。从妊娠第10天到第11天，胚胎葡萄糖氧化略有下降，且在这两天糖尿病大鼠胚胎的葡萄糖氧化率均低于正常大鼠胚胎。胚胎中的乳酸生成量和含量随孵育培养基中葡萄糖浓度的增加而增加，而葡萄糖氧化不受葡萄糖浓度影响。结果表明，葡萄糖利用率的变化对胚胎期葡萄糖代谢的影响很小。即使将这些代谢变量的值按胚胎DNA含量进行标准化，糖尿病大鼠胚胎的乳酸生成率仍高于正常大鼠胚胎，而葡萄糖氧化率则低于正常大鼠胚胎。因此，可以设想葡萄糖代谢紊乱在糖尿病诱导的胚胎畸形发生中起因果作用。
