WEIGLE W O
J Exp Med. 1962 Dec 1;116(6):913-28. doi: 10.1084/jem.116.6.913.
Acquired tolerance to BSA in rabbits was terminated following the injection of certain preparations of altered BSA. Injections of Freund's adjuvant containing BSA complexed to anti-BSA, heat-denatured BSA, acetyl-BSA, picryl-BSA, or arsanil-BSA failed to terminate the tolerant state. Except in an occasional tolerant rabbit, injections of these preparations failed to cause the production of precipitating antibody to the altered preparation. Similar results were obtained following injections of alum-precipitated preparations of pepsin-degraded BSA, acetyl-BSA, and picryl-BSA. Injections of Freund's adjuvant containing sulfanil-BSA terminated the tolerant state, but only small amounts of non-precipitating anti-BSA were produced. Injections of Freund's adjuvant containing picryl-acetyl-BSA terminated the tolerant state in two of six rabbits, but again, only small amounts of non-precipitating anti-BSA were produced. Injections of an alum-precipitated preparation of picryl-acetyl-BSA failed to terminate the tolerant state. On the other hand, injections of Freund's adjuvant containing arsanil-sulfanil-BSA terminated the tolerant state in eleven of eleven rabbits and caused the production of precipitating anti-BSA in all nine of the rabbits tested. The tolerant state was terminated also in six of six rabbits injected with an alum-precipitated preparation of arsanil-sulfanil-BSA. Only one of these rabbits produced precipitating anti-BSA. In addition, the injection of BGG-tolerant rabbits with arsanil-BGG, sulfanil-BGG, or arsanil-sulfanil-BGG terminated the tolerant state. These results were discussed in relation to both the clonal selection theory of antibody production and autoimmunity.
给家兔注射某些经改变的牛血清白蛋白（BSA）制剂后，其获得性对BSA的耐受性被终止。注射含有与抗BSA复合的BSA、热变性BSA、乙酰化BSA、苦味酸BSA或对氨基苯胂酸BSA的弗氏佐剂未能终止耐受状态。除了偶尔有一只耐受的家兔外，注射这些制剂未能引起针对经改变制剂的沉淀抗体的产生。注射胃蛋白酶降解的BSA、乙酰化BSA和苦味酸BSA的明矾沉淀制剂后也得到了类似的结果。注射含有对氨基苯磺酰胺BSA的弗氏佐剂终止了耐受状态，但只产生了少量的非沉淀性抗BSA。注射含有苦味酸乙酰化BSA的弗氏佐剂使6只家兔中的2只终止了耐受状态，但同样只产生了少量的非沉淀性抗BSA。注射苦味酸乙酰化BSA的明矾沉淀制剂未能终止耐受状态。另一方面，注射含有对氨基苯胂酸对氨基苯磺酰胺BSA的弗氏佐剂使11只家兔中的11只终止了耐受状态，并使所有接受测试的9只家兔产生了沉淀性抗BSA。注射对氨基苯胂酸对氨基苯磺酰胺BSA的明矾沉淀制剂也使6只家兔中的6只终止了耐受状态。这些家兔中只有一只产生了沉淀性抗BSA。此外，给对牛γ球蛋白（BGG）耐受的家兔注射对氨基苯胂酸BGG、对氨基苯磺酰胺BGG或对氨基苯胂酸对氨基苯磺酰胺BGG也终止了耐受状态。结合抗体产生的克隆选择理论和自身免疫对这些结果进行了讨论。
