Tsai H J, Saif Y M, Nestor K E, Emmerson D A, Patterson R A
Food Animal Health Research Program, Ohio State University, Wooster 44691.
Avian Dis. 1992 Jul-Sep;36(3):561-5.
Seven hundred eighty male and female turkeys representing four genetic lines were challenged in four experiments with the Texas GB strain of Newcastle disease virus (NDV). The lines of turkeys included two randombred control lines (RBC1 and RBC2), a subline (E) of RBC1 selected for increased egg production, and a subline (F) of RBC2 selected for increased 16-week body weight. Mortality in turkeys of subline F (32.5%) was significantly higher than that in turkeys of line RBC2 (15.8%), subline E (17.5%), and line RBC1 (18.4%). At the end of each experiment, surviving birds were tested for antibody to NDV using the enzyme-linked immunosorbent assay (ELISA) and hemagglutination-inhibition (HI) test. Turkeys of subline E and line RBC1 had significantly lower ELISA antibody titers than those of subline F and line RBC2. Subline F had the highest HI antibody titers, followed in decreasing order by lines RBC2 and RBC1 and subline E. No apparent correlation was found between antibody response and mortality after NDV challenge.
在四项实验中，用得克萨斯GB株新城疫病毒（NDV）对代表四个遗传品系的780只雌雄火鸡进行了攻毒试验。火鸡品系包括两个随机交配对照品系（RBC1和RBC2）、为提高产蛋量而选择的RBC1的一个亚系（E）以及为提高16周龄体重而选择的RBC2的一个亚系（F）。亚系F火鸡的死亡率（32.5%）显著高于RBC2品系（15.8%）、亚系E（17.5%）和RBC1品系（18.4%）的火鸡。在每项实验结束时，对存活的火鸡使用酶联免疫吸附测定（ELISA）和血凝抑制（HI）试验检测新城疫病毒抗体。亚系E和RBC1品系的火鸡ELISA抗体效价显著低于亚系F和RBC2品系的火鸡。亚系F的HI抗体效价最高，其次依次是RBC2品系、RBC1品系和亚系E。在新城疫病毒攻毒后，未发现抗体反应与死亡率之间存在明显相关性。
