Xiao L, Celano P, Mank A R, Griffin C, Jabs E W, Hawkins A L, Casero R A
Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231.
Biochem Biophys Res Commun. 1992 Sep 30;187(3):1493-502. doi: 10.1016/0006-291x(92)90471-v.
The super induction of spermidine/spermine N1-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a "TATA-less" promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.
亚精胺/精胺N1 - 乙酰基转移酶（SSAT）的超诱导作用与人类实体瘤对双（乙基）多胺的细胞毒性反应有关。SSAT反应是一种表型特异性反应，在稳态mRNA水平增加和酶蛋白水平上受到调节。包含SSAT编码序列的人类基因组区域（4095个碱基）已被克隆并定位到Xp22.1区域。引物延伸分析表明，SSAT的转录起始于翻译起始位点上游179个碱基处，并且似乎受一个“无TATA”启动子的控制。这个人类克隆的获得将有助于对SSAT基因进行直接的功能研究。
