Saijonmaa O, Nyman T, Fyhrquist F
Minerva Institute for Medical Research, Helsinki, Finland.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):286-91. doi: 10.1016/0006-291x(92)92382-8.
We studied whether endothelin-1 (ET-1) would affect its own synthesis. Human umbilical cord vein endothelial cells in methionine-poor culture medium containing [35S] methionine were treated with synthetic ET-1 or ET-3. Immunoprecipitation of 35 S-labeled ET-1 was performed with rabbit ET-1 antiserum. ET-1 caused an 40 +/- 4% (mean +/- SEM) increase of immunoprecipitable 35 S-labeled ET-1 as confirmed by its elution point in reversed phase high power liquid chromatography (HPLC). ET-3 caused a 23 +/- 2% increase in ET-1 concentration. Amplification of cDNA by PCR showed both ET-1 and ETB receptor mRNAs in human cord vein endothelial cells. We conclude that ET-1 increases its own synthesis in endothelial cells. This suggests a positive autocrine feed-back action of ET-1 on its own synthesis, an effect which is probably mediated by non-specific ETB receptors.
我们研究了内皮素-1（ET-1）是否会影响其自身的合成。将含有[35S]蛋氨酸的低蛋氨酸培养基中的人脐静脉内皮细胞用合成的ET-1或ET-3进行处理。用兔ET-1抗血清对35S标记的ET-1进行免疫沉淀。通过反相高效液相色谱（HPLC）中的洗脱点证实，ET-1使可免疫沉淀的35S标记的ET-1增加了40±4%（平均值±标准误）。ET-3使ET-1浓度增加了23±2%。通过PCR扩增cDNA显示人脐静脉内皮细胞中存在ET-1和ETB受体mRNA。我们得出结论，ET-1在内皮细胞中增加其自身的合成。这表明ET-1对其自身合成具有正性自分泌反馈作用，这种作用可能由非特异性ETB受体介导。
