Yoshida H, Nakamura M, Makita S, Hiramori K
Second Department of Internal Medicine, Iwate Medical University, Japan.
Heart Vessels. 1996;11(5):229-33. doi: 10.1007/BF01746202.
To investigate the role endothelial cells have on underlying smooth muscle cell proliferation, human aortic vascular smooth muscle cells were co-cultured with human aortic endothelial cells at different cell densities, using a transmembrane co-culture method. Subconfluent endothelial cells subseeded at low (0.5 x 10(4) cells/well) and medium densities (2.0 x 10(4) cells/well) stimulated smooth muscle cell proliferation by 43 +/- 14% (P < 0.01) and 39 +/- 8% (P < 0.02), respectively. However, this stimulatory effect on smooth muscle cell proliferation was not evident in confluent endothelial cells subseeded at high cell density (8.0 x 10(4) cells/well). Treatment of smooth muscle cells with trapidil, at 10(-6) M, for anti-platelet derived growth factor (PDGF) effect or with endothelin-1 receptor blocker FR 139317, at 10(-6) M failed to inhibit this stimulatory effect. These results imply that subconfluent human endothelial cells are able to exert a stimulatory effect on human smooth muscle cell proliferation, and that this endothelial paracrine growth effect may not be mediated by endothelin or PDGF.
为了研究内皮细胞在其下方平滑肌细胞增殖中所起的作用，采用跨膜共培养方法，将人主动脉血管平滑肌细胞与人主动脉内皮细胞以不同细胞密度进行共培养。以低（0.5×10⁴个细胞/孔）和中等密度（2.0×10⁴个细胞/孔）接种的亚汇合内皮细胞分别刺激平滑肌细胞增殖43±14%（P<0.01）和39±8%（P<0.02）。然而，以高细胞密度（8.0×10⁴个细胞/孔）接种的汇合内皮细胞对平滑肌细胞增殖的这种刺激作用并不明显。用10⁻⁶M的曲匹地尔处理平滑肌细胞以发挥抗血小板衍生生长因子（PDGF）的作用，或用10⁻⁶M的内皮素-1受体阻滞剂FR 139317处理，均未能抑制这种刺激作用。这些结果表明，亚汇合的人内皮细胞能够对人平滑肌细胞增殖发挥刺激作用，并且这种内皮旁分泌生长作用可能不是由内皮素或PDGF介导的。
