Moriyama T, Fujiwara Y, Imai E, Takenaka M, Kawanishi S, Inoue T, Noguchi T, Tanaka T, Kamada T, Ueda N
1st Department of Medicine, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):34-9. doi: 10.1016/0006-291x(92)92346-y.
Protein tyrosine phosphatases (PTPases) are a family of enzymes that play a crucial role in the regulation of signal transduction mediated by reversible protein tyrosine phosphorylation. To understand the significance of PTPases in physiological and pathophysiological processes in the kidney, we isolated three cDNA segments encoding PTPases (LAR, LRP and a novel PTPase) from rat kidney by polymerase chain reaction (PCR). Using PCR product as a probe, we isolated a full-length cDNA of rat LRP. LRP cDNA encoded a single membrane spanning protein consisted of 796 amino acids, with two tandemly located intracellular PTPase domains. By Northern analysis, a ubiquitous pattern of LRP gene expression in rat tissues was demonstrated. In cultured rat mesangial cells, LRP mRNA was detected and the mRNA level was suppressed by either interleukin-1 or interleukin-6 treatment.
蛋白酪氨酸磷酸酶（PTPases）是一类在由可逆性蛋白酪氨酸磷酸化介导的信号转导调节中起关键作用的酶。为了解PTPases在肾脏生理和病理生理过程中的意义，我们通过聚合酶链反应（PCR）从大鼠肾脏中分离出三个编码PTPases的cDNA片段（LAR、LRP和一种新型PTPase）。以PCR产物为探针，我们分离出了大鼠LRP的全长cDNA。LRP cDNA编码一种由796个氨基酸组成的单跨膜蛋白，具有两个串联的细胞内PTPase结构域。通过Northern分析，证实了LRP基因在大鼠组织中的普遍表达模式。在培养的大鼠系膜细胞中，检测到了LRP mRNA，并且白细胞介素-1或白细胞介素-6处理可抑制其mRNA水平。
