• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

血小板衍生生长因子A链启动子中一个对S1核酸酶敏感的同型嘌呤/同型嘧啶结构域包含生长因子诱导蛋白EGR-1的一个新结合位点。

An S1 nuclease-sensitive homopurine/homopyrimidine domain in the PDGF A-chain promoter contains a novel binding site for the growth factor-inducible protein EGR-1.

作者信息

Wang Z Y, Deuel T F

机构信息

Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.

出版信息

Biochem Biophys Res Commun. 1992 Oct 15;188(1):433-9. doi: 10.1016/0006-291x(92)92403-k.

DOI:10.1016/0006-291x(92)92403-k
PMID:1417865
Abstract

Transcription of the platelet-derived growth factor (PDGF) A-chain gene is activated in cells exposed to growth factors. We now have identified a homopurine/homopyrimidine domain in the promoter region of the PDGF A-chain gene that exhibits S1 nuclease sensitivity in vitro and that contains a novel binding site (5'-TCCTCCTCCTCCTC-3') for the growth factor inducible transcription factor EGR-1 as demonstrated in gel mobility shift assays. Sequences similar to this novel EGR-1 binding site were observed also in five growth-related genes and shown to bind to EGR-1 in competition assays, suggesting that EGR-1 may influence the transcriptional regulation of these growth-related genes.

摘要

血小板衍生生长因子（PDGF）A链基因的转录在暴露于生长因子的细胞中被激活。我们现已在PDGF A链基因的启动子区域鉴定出一个同嘌呤/同嘧啶结构域，该结构域在体外表现出对S1核酸酶敏感，并且含有一个新的生长因子诱导转录因子EGR-1的结合位点（5'-TCCTCCTCCTCCTC-3'），凝胶迁移率变动分析证明了这一点。在五个与生长相关的基因中也观察到与这个新的EGR-1结合位点相似的序列，并且在竞争分析中显示它们能与EGR-1结合，这表明EGR-1可能影响这些与生长相关基因的转录调控。

相似文献

1
An S1 nuclease-sensitive homopurine/homopyrimidine domain in the PDGF A-chain promoter contains a novel binding site for the growth factor-inducible protein EGR-1.血小板衍生生长因子A链启动子中一个对S1核酸酶敏感的同型嘌呤/同型嘧啶结构域包含生长因子诱导蛋白EGR-1的一个新结合位点。
Biochem Biophys Res Commun. 1992 Oct 15;188(1):433-9. doi: 10.1016/0006-291x(92)92403-k.
2
Inducible PDGF A-chain transcription in smooth muscle cells is mediated by Egr-1 displacement of Sp1 and Sp3.平滑肌细胞中诱导型血小板衍生生长因子A链转录由Egr-1取代Sp1和Sp3介导。
Am J Physiol. 1997 Sep;273(3 Pt 2):H1415-26. doi: 10.1152/ajpheart.1997.273.3.H1415.
3
Modulation of transcription of the platelet-derived growth factor A-chain gene by a promoter region sensitive to S1 nuclease.对S1核酸酶敏感的启动子区域对血小板衍生生长因子A链基因转录的调控
J Biol Chem. 1992 Aug 25;267(24):17022-31.
4
An S1 nuclease sensitive region in the PDGFA-chain gene promoter contains a positive transcriptional regulatory element.血小板衍生生长因子A链基因启动子中的一个对S1核酸酶敏感的区域含有一个正向转录调节元件。
Biochem Biophys Res Commun. 1994 Jan 14;198(1):103-10. doi: 10.1006/bbrc.1994.1015.
5
Lysophosphatidylcholine induces early growth response factor-1 expression and activates the core promoter of PDGF-A chain in vascular endothelial cells.溶血磷脂酰胆碱诱导血管内皮细胞中早期生长反应因子-1的表达并激活血小板衍生生长因子-A链的核心启动子。
Arterioscler Thromb Vasc Biol. 2001 May;21(5):771-6. doi: 10.1161/01.atv.21.5.771.
6
Identification of a single-stranded DNA-binding protein that interacts with an S1 nuclease-sensitive region in the platelet-derived growth factor A-chain gene promoter.一种与血小板衍生生长因子A链基因启动子中S1核酸酶敏感区域相互作用的单链DNA结合蛋白的鉴定。
J Biol Chem. 1993 May 15;268(14):10681-5.
7
Functional role of a conformationally flexible homopurine/homopyrimidine domain of the androgen receptor gene promoter interacting with Sp1 and a pyrimidine single strand DNA-binding protein.雄激素受体基因启动子中一个构象灵活的同型嘌呤/同型嘧啶结构域与Sp1及一种嘧啶单链DNA结合蛋白相互作用的功能作用。
Mol Endocrinol. 1997 Jan;11(1):3-15. doi: 10.1210/mend.11.1.9868.
8
Egr-1 is activated in endothelial cells exposed to fluid shear stress and interacts with a novel shear-stress-response element in the PDGF A-chain promoter.Egr-1在暴露于流体剪切应力的内皮细胞中被激活，并与血小板衍生生长因子A链启动子中的一个新的剪切应力反应元件相互作用。
Arterioscler Thromb Vasc Biol. 1997 Oct;17(10):2280-6. doi: 10.1161/01.atv.17.10.2280.
9
Structural and functional characterization of the human decorin gene promoter. A homopurine-homopyrimidine S1 nuclease-sensitive region is involved in transcriptional control.人核心蛋白聚糖基因启动子的结构与功能特性。一个同型嘌呤-同型嘧啶S1核酸酶敏感区域参与转录调控。
J Biol Chem. 1994 Jan 7;269(1):579-87.
10
An S1 nuclease-sensitive region in the first intron of human platelet-derived growth factor A-chain gene contains a negatively acting cell type-specific regulatory element.人血小板衍生生长因子A链基因第一内含子中的一个S1核酸酶敏感区域含有一个负向作用的细胞类型特异性调控元件。
Nucleic Acids Res. 1994 Feb 11;22(3):457-64. doi: 10.1093/nar/22.3.457.

引用本文的文献

1
A microarray analysis of retinal transcripts that are controlled by image contrast in mice.对受小鼠图像对比度控制的视网膜转录本进行的微阵列分析。
Mol Vis. 2007 Jun 18;13:920-32.
2
The transcription factor early growth response factor-1 (EGR-1) promotes apoptosis of neuroblastoma cells.转录因子早期生长反应因子-1（EGR-1）可促进神经母细胞瘤细胞的凋亡。
Biochem J. 2003 Aug 1;373(Pt 3):739-46. doi: 10.1042/BJ20021918.
3
Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
早期生长反应蛋白（EGR）和活化T细胞核因子（NFAT）形成异二聚体并调节促炎细胞因子基因表达。
Nucleic Acids Res. 2003 Feb 1;31(3):911-21. doi: 10.1093/nar/gkg186.
4
Cyclosporin A-sensitive transcription factor Egr-3 regulates Fas ligand expression.环孢菌素A敏感转录因子Egr-3调节Fas配体的表达。
Mol Cell Biol. 1998 Jul;18(7):3744-51. doi: 10.1128/MCB.18.7.3744.
5
Structural and functional analysis of a promoter of the human granulin/epithelin gene.人类颗粒蛋白/上皮生成素基因启动子的结构与功能分析
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):441-7. doi: 10.1042/bj3190441.
6
Transcription factor EGR-1 suppresses the growth and transformation of human HT-1080 fibrosarcoma cells by induction of transforming growth factor beta 1.转录因子EGR-1通过诱导转化生长因子β1抑制人HT-1080纤维肉瘤细胞的生长和转化。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11831-6. doi: 10.1073/pnas.93.21.11831.
7
Signals controlling the expression of PDGF.控制血小板源性生长因子（PDGF）表达的信号。
Mol Biol Rep. 1995;22(1):1-24. doi: 10.1007/BF00996300.
8
The immediate-early gene Egr-1 regulates the activity of the thymidine kinase promoter at the G0-to-G1 transition of the cell cycle.即早基因Egr-1在细胞周期的G0到G1转变过程中调节胸苷激酶启动子的活性。
Mol Cell Biol. 1994 Aug;14(8):5242-8. doi: 10.1128/mcb.14.8.5242-5248.1994.
9
Role of EGR-1 in thapsigargin-inducible apoptosis in the melanoma cell line A375-C6.早期生长反应因子-1（EGR-1）在毒胡萝卜素诱导的黑色素瘤细胞系A375-C6凋亡中的作用
Mol Cell Biol. 1995 Nov;15(11):6262-72. doi: 10.1128/MCB.15.11.6262.