Wiig H, Heir S, Aukland K
Department of Physiology, University of Bergen, Norway.
Acta Physiol Scand. 1988 Jun;133(2):167-75. doi: 10.1111/j.1748-1716.1988.tb08396.x.
Interstitial fluid for measurement of colloid osmotic pressure (COPi) was collected from rat subcutis and skeletal muscle with three wick methods; saline-soaked wicks implanted in vivo, titration with wicks preloaded with serial dilutions of rat plasma implanted postmortem (crossover method) and dry wicks implanted in vivo and post-mortem. When compared in 12 rats, the mean subcutaneous COPi of 14.2 and 13.3 mmHg as estimated with the crossover method and dry wick method, respectively, both exceeded the 10.7 mmHg obtained in saline-soaked wicks implanted for 60 min. Colloid osmotic pressure (COP) in fluid from dry wicks implanted subcutaneously was slightly lower than in plasma 5 min after implantation in vivo, then fell gradually, but remained stable the last 30 min of the 60 min in vivo implantation period and the first 30 min post-mortem, when again starting to rise. COP in fluid isolated from dry wicks implanted in muscle post-mortem approached or even exceeded that of plasma, suggesting leakage of intracellular proteins to wick fluid. Electrophoresis of dry wick fluid from subcutis showed a pattern similar to that of plasma, while protein fractions not present in plasma were observed in muscle wick fluid. For measurement of COPi we recommend the use of dry wicks implanted post mortem for subcutis and saline-soaked or dry wicks implanted in vivo for 90-120 min for skeletal muscle.
采用三种灯芯法从大鼠皮下组织和骨骼肌中采集用于测量胶体渗透压（COPi）的组织间液；将浸泡生理盐水的灯芯植入体内、用死后植入预先加载不同稀释度大鼠血浆的灯芯进行滴定（交叉法）以及将干灯芯在体内和死后植入。在12只大鼠中进行比较时，交叉法和干灯芯法估计的皮下平均COPi分别为14.2 mmHg和13.3 mmHg，均超过了浸泡生理盐水60分钟的灯芯所测得的10.7 mmHg。皮下植入干灯芯后5分钟，其液体中的胶体渗透压（COP）略低于血浆，随后逐渐下降，但在体内植入60分钟的最后30分钟和死后的前30分钟保持稳定，之后又开始上升。死后从肌肉植入的干灯芯分离出的液体中的COP接近甚至超过血浆，提示细胞内蛋白质漏入灯芯液体。皮下干灯芯液体的电泳显示出与血浆相似的模式，而在肌肉灯芯液体中观察到血浆中不存在的蛋白质组分。对于COPi的测量，我们建议皮下使用死后植入的干灯芯，骨骼肌使用体内植入90 - 120分钟的浸泡生理盐水或干灯芯。
