BARIL E F, LOVE D S, HERRMANN H
Science. 1964 Oct 16;146(3642):413-4. doi: 10.1126/science.146.3642.413.
Chromatography, on diethylaminoethyl cellulose, of leg muscle extracts from older chick embryos yields three myosin-containing fractions, whereas material from muscle extracts of 11-day embryos cannot be eluted under the same conditions. A major fraction having the adenosine triphosphotase activity characteristic of myosin can be eluted when 11-day-old embryo muscle extracts are given prior treatment with ribonuclease.
用二乙氨基乙基纤维素对老龄鸡胚腿部肌肉提取物进行层析，可得到三个含肌球蛋白的组分，而在相同条件下，11日龄胚胎肌肉提取物中的物质无法洗脱。当用核糖核酸酶对11日龄胚胎肌肉提取物进行预处理后，具有肌球蛋白特征性三磷酸腺苷酶活性的主要组分能够被洗脱。
