Nagano T, Kita T, Tanaka N
Department of Legal Medicine, Kanazawa Medical University, Japan.
Int J Exp Pathol. 1992 Oct;73(5):675-83.
After administration of bacterial lipopolysaccharide, there is an increase in the number of leucocytes which adhere to the endothelial cell surface of the hepatic vessels and pass through the endothelial layer by comparison with controls. There is also marked endothelial cell damage including intracytoplasmic oedema, increased numbers of autophagic vacuoles and dilatation of the intercellular junction in LPS-treated samples. The presence of immunocytochemical products of leukotriene (LTR) and tumour necrosis factor (TNF) was examined using in both LPS-treated and control samples. Immunoreactions of LTR which were seen in specific granules of neutrophils and monocytes attached to the endothelial cell surface may indicate the onset of endothelial cell damage. Positive immunoreactions of TNF on the endothelial cell surface, seen only in LPS-treated samples, indicate that TNF may enhance the passage of blood cells through the endothelia and also increase the endocytotic activity of the liver parenchymal cells, as revealed by the present marker experiment using horseradish peroxidase. Positive reactions of TNF in lysosomes of the endothelial cells suggest that they are able to produce TNF and transport it to the cell surface.
给予细菌脂多糖后，与对照组相比，粘附于肝血管内皮细胞表面并穿过内皮层的白细胞数量增加。在脂多糖处理的样本中，还存在明显的内皮细胞损伤，包括胞浆水肿、自噬泡数量增加和细胞间连接扩张。在脂多糖处理组和对照组样本中均检测了白三烯（LTR）和肿瘤坏死因子（TNF）的免疫细胞化学产物的存在情况。在内皮细胞表面附着的中性粒细胞和单核细胞的特异性颗粒中观察到的LTR免疫反应可能表明内皮细胞损伤的开始。仅在脂多糖处理的样本中在内皮细胞表面观察到的TNF阳性免疫反应表明，TNF可能增强血细胞穿过内皮的能力，并且如使用辣根过氧化物酶的当前标记实验所揭示的，还可增加肝实质细胞的内吞活性。内皮细胞溶酶体中TNF的阳性反应表明它们能够产生TNF并将其转运至细胞表面。
