A型肉毒杆菌低分子量毒性成分的分离与鉴定

ISOLATION AND CHARACTERIZATION OF A TOXIC MOIETY OF LOW MOLECULAR WEIGHT FROM CLOSTRIDIUM BOTULINUM TYPE A.

作者信息

GERWING J, DOLMAN C E, BAINS H S

出版信息

J Bacteriol. 1965 May;89(5):1383-6. doi: 10.1128/jb.89.5.1383-1386.1965.

DOI:10.1128/jb.89.5.1383-1386.1965

PMID:14293025

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277655/

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, and Hardial S. Bains. Isolation and characterization of a toxic moiety of low molecular weight from Clostridium botulinum type A. J. Bacteriol. 89:1383-1386. 1965.-A toxic moiety of low molecular weight has been isolated from a type A strain of Clostridium botulinum, by a method involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. By means of electrophoresis and ultracentrifugation, the toxic substance was shown to be homogeneous; a molecular weight of 12,200 was calculated.

摘要

格温，朱莉娅（加拿大不列颠哥伦比亚省温哥华市英属哥伦比亚大学），克劳德·E·多尔曼，以及哈迪尔·S·贝恩斯。从A型肉毒梭菌中分离和鉴定低分子量有毒部分。《细菌学杂志》89:1383 - 1386。1965年。——通过一种涉及硫酸铵沉淀以及在pH 5.6条件下通过二乙氨基乙基纤维素洗脱的方法，从一株A型肉毒梭菌中分离出了一种低分子量有毒部分。通过电泳和超速离心法，表明该有毒物质是均一的；计算出其分子量为12200。