CHERRY J H
Science. 1964 Nov 20;146(3647):1066-9. doi: 10.1126/science.146.3647.1066.
An association of rapidly metabolized DNA and RNA labeled with P(32) has been found in peanut cotyledons by incorporation of either uridine-C(14) or thymidine-C(14) by peanut cotyledon slices. Chromatography of the extracted nucleic acids on methylated- albumin columns shows that the rapidly metabolized DNA and RNA are contained only in the first half of the DNA peak where RNA and DNA occur in equal amount. One-fourth of the radioactivity is recovered in ribonuc'eotides. Density-gradient centrifugation in cesium chloride indicates that the material containing the P(32) has a higher density than most of the DNA.
通过花生子叶切片掺入尿苷-C(14) 或胸苷-C(14),已在花生子叶中发现了与用P(32)标记的快速代谢的DNA和RNA的关联。在甲基化白蛋白柱上对提取的核酸进行色谱分析表明,快速代谢的DNA和RNA仅存在于DNA峰的前半部分,其中RNA和DNA含量相等。四分之一的放射性在核糖核苷酸中回收。在氯化铯中进行密度梯度离心表明,含有P(32)的物质的密度高于大多数DNA。