氟乐灵对生长、形态和核酸合成的影响。

Effect of trifluralin on growth, morphology, and nucleic Acid synthesis.

机构信息

Department of Botany and Plant Pathology, Auburn University, Auburn, Alabama.

出版信息

Plant Physiol. 1968 Feb;43(2):265-73. doi: 10.1104/pp.43.2.265.

DOI:10.1104/pp.43.2.265

PMID:16656762

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1086829/

Abstract

Roots and shoots of corn seedlings (Zea mays L. var. Dixie 18) germinated in trifluralin (alpha,alpha,alpha-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) solutions are characterized by radial enlargement of the cortical cells and by multinucleate cells in the meristematic regions. Trifluralin inhibits elongation of Avena coleoptile sections at concentrations of 0.1 mum to 10 mum. Synthesis of DNA, RNA, and protein is suppressed in the root tips while no significant effect is noticeable in the shoots of corn germinated in trifluralin. A (32)P time-course study of 48, 72, and 96 hours utilizing phenol extraction and MAK column separation of corn root and shoot nucleic acids showed suppression of (32)P incorporation in the treated roots; however, the 72 and 96 hour treated shoots incorporated a much greater amount than the control with most of the increased incorporation found in the sRNA and DNA fractions. The increased activity in the DNA may be due to a high G-C type DNA. No selective suppression or enhancement of any particular RNA species was noticed in the treated plants.

摘要

玉米幼苗（Zea mays L. var. Dixie 18）的根和芽在氟乐灵（α,α,α-三氟-2,6-二硝基-N,N-二丙基-对甲苯胺）溶液中萌发的特征是皮质细胞的径向扩大和分生组织区域的多核细胞。氟乐灵在 0.1 mum 至 10 mum 的浓度下抑制燕麦胚芽鞘切段的伸长。在玉米芽中，DNA、RNA 和蛋白质的合成受到抑制，而在根中则没有明显的影响。利用苯酚提取和玉米根和芽核酸的 MAK 柱分离，对 48、72 和 96 小时进行 (32)P 时程研究表明，处理过的根中 (32)P 掺入受到抑制；然而，72 和 96 小时处理过的芽比对照吸收了更多的 (32)P，大部分增加的掺入发生在 sRNA 和 DNA 中。DNA 中的活性增加可能是由于高 GC 型 DNA。在处理过的植物中没有发现任何特定 RNA 种类的选择性抑制或增强。