胸腺嘧啶、胸腺嘧啶核苷、尿嘧啶、尿苷和5-氟尿嘧啶在枯草芽孢杆菌核酸中的摄取与掺入
UPTAKE AND INCORPORATION OF THYMINE, THYMIDINE, URACIL, URIDINE, AND 5-FLUOROURACIL INTO THE NUCLEIC ACIDS OF BACILLUS SUBTILIS.
作者信息
BODMER W F, GRETHER S
出版信息
J Bacteriol. 1965 Apr;89(4):1011-4. doi: 10.1128/jb.89.4.1011-1014.1965.
Bodmer, Walter F. (Stanford University School of Medicine, Palo Alto, Calif.), and Susan Grether. Uptake and incorporation of thymine, thymidine, uracil, uridine, and 5-fluorouracil into the nucleic acids of Bacillus subtilis. J. Bacteriol. 89:1011-1014. 1965.-From 55 to 95% of uracil, uridine, or 5-fluorouracil (FU) added to the culture medium is incorporated into the acid-insoluble fraction of cells of Bacillus subtilis strains SB 19 (prototroph) and SB 503 (FU-resistant). Thymine is poorly incorporated (less than 1%); thus, the incorporation of thymidine is limited (less than 12%) by the rapid degradation of the nucleoside to thymine, probably by the enzyme thymidine phosphorylase. Uracil, uridine, and FU were not incorporated into the deoxyribonucleic acid (DNA) of either strain, whereas all the incorporated thymidine was found in the DNA.
博德默，沃尔特·F.（加利福尼亚州帕洛阿尔托斯坦福大学医学院）和苏珊·格雷瑟。胸腺嘧啶、胸腺嘧啶核苷、尿嘧啶、尿嘧啶核苷和5-氟尿嘧啶被枯草芽孢杆菌核酸摄取和掺入的情况。《细菌学杂志》89:1011 - 1014。1965年。 - 添加到培养基中的尿嘧啶、尿嘧啶核苷或5-氟尿嘧啶（FU）有55%至95%被掺入枯草芽孢杆菌菌株SB 19（原养型）和SB 503（耐FU）细胞的酸不溶性部分。胸腺嘧啶掺入量很少（不到1%）；因此，胸腺嘧啶核苷的掺入受到限制（不到12%），因为该核苷迅速降解为胸腺嘧啶，可能是由胸腺嘧啶磷酸化酶催化。尿嘧啶、尿嘧啶核苷和FU均未掺入任何一种菌株的脱氧核糖核酸（DNA）中，而所有掺入的胸腺嘧啶核苷都存在于DNA中。
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