Cametti C, De Luca F, D'Ilario A, Macrì M A, Maraviglia B, Bordi F, Lenti L, Misasi R, Sorice M
Dipartimento di Fisica, Università La Sapienza, Roma, Italy.
Biochim Biophys Acta. 1992 Nov 9;1111(2):197-203. doi: 10.1016/0005-2736(92)90311-9.
The electrical conductivity of normal human lymphocyte suspensions has been measured in the frequency range from 10 kHz to 100 MHz, where a well-pronounced conductivity dispersion occurs, caused by the surface polarization at the interface between the cell membrane and the extracellular solution. We have investigated the alteration of the passive electrical properties of the cytoplasmatic cell membrane induced by two different gangliosides (GM1 and GM3) inserted, at various concentrations, into the outer leaflet of membrane double layer. The alterations observed in the dielectric parameters (the membrane conductivity and the membrane permittivity) derived on the basis of a 'double-shell' model, result in an overall increase of the ion permeation across the membrane and an enhanced polarizability of its hydrophilic region for both gangliosides investigated. The relevance of these alterations is discussed.
已在10 kHz至100 MHz的频率范围内测量了正常人淋巴细胞悬液的电导率，在此频率范围内，由于细胞膜与细胞外溶液界面处的表面极化，出现了明显的电导率色散。我们研究了两种不同的神经节苷脂（GM1和GM3）以不同浓度插入膜双层外小叶后，细胞质细胞膜被动电学性质的变化。基于“双壳”模型得出的介电参数（膜电导率和膜电容率）的变化，导致所研究的两种神经节苷脂跨膜离子渗透总体增加，其亲水区域的极化率增强。讨论了这些变化的相关性。
