Uber-Bucek E, Hamon M, Pham Huy C, Dadoun H
Laboratoire de Pharmacognosie, Faculté des Sciences, Chatenay Malabry, France.
J Pharm Biomed Anal. 1992 Jun;10(6):413-9. doi: 10.1016/0731-7085(92)80059-v.
Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay. Anti-digitoxin antibody was used, thevetin B genin being structurally identical to digitoxigenin. Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1.
用荧光偏振免疫分析法测定了夹竹桃科植物黄花夹竹桃种子中的强心苷黄夹次苷B在血清中的含量。使用了抗洋地黄毒苷抗体，黄夹次苷B配基在结构上与洋地黄毒苷元相同。用该方法测定5至80纳克/毫升浓度范围内的黄夹次苷B时，交叉反应率为94%。
