Banks R W, Harker D W, Stacey M J
J Anat. 1977 Jul;123(Pt 3):783-96.
A direct correlation of the histochemical and ultrastructural properties of intrafusal muscle fibres has been achieved by cutting frozen serial sections for histochemical applications (15 micron thick sections) and for electron microscopy (60 micron thick sections) in a repeating sequence. Three types of intrafusal fibre were recognized, including two types of nuclear-bag fibre, designated bag1 and bag2. In addition to histochemical and ultrastructural differences, the three types of fibre differed in length and diameter. Regional variations of histochemical and ultrastructural properties were found. The results are compared with previous attempts to correlate histochemical and ultrastructural properties of intrafusal muscle fibres based on indirect methods.
通过按重复顺序切割用于组织化学应用(15微米厚切片)和电子显微镜检查(60微米厚切片)的冷冻连续切片,已实现了对梭内肌纤维组织化学和超微结构特性的直接关联。识别出了三种类型的梭内纤维,包括两种类型的核袋纤维,分别称为袋1和袋2。除了组织化学和超微结构差异外,这三种纤维在长度和直径上也有所不同。还发现了组织化学和超微结构特性的区域差异。将这些结果与先前基于间接方法对梭内肌纤维组织化学和超微结构特性进行关联的尝试进行了比较。