Leoni S, Spagnuolo S, Massimi M, Terenzi F, Conti Devirgiliis L
Dept. of Cellular and Developmental Biology, University La Sapienza, Rome, Italy.
Biosci Rep. 1992 Apr;12(2):135-41. doi: 10.1007/BF02351218.
Amino acid uptake mediated by system A was studied in cultured fetal and adult hepatocytes, subjected to growth stimulation by EGF and insulin, or to growth inhibition by high cell density. The mitogenic stimulation induced a strong transport increase only in fetal cells, while the cell density-dependent growth inhibition, probably mediated by molecules present on adult hepatocyte membranes, provoked the decrease of amino acid uptake only in the adult cells. The results indicate that the different modulation of amino acid transport by cell growth is dependent on the age and the differentiation stage of hepatocytes.
在培养的胎儿和成年肝细胞中研究了由A系统介导的氨基酸摄取，这些细胞受到表皮生长因子（EGF）和胰岛素的生长刺激，或高细胞密度的生长抑制。有丝分裂刺激仅在胎儿细胞中诱导了强烈的转运增加，而可能由成年肝细胞膜上存在的分子介导的细胞密度依赖性生长抑制，仅在成年细胞中引起氨基酸摄取的减少。结果表明，细胞生长对氨基酸转运的不同调节取决于肝细胞的年龄和分化阶段。
