Napier J A, Larsson K H, Madueño F, Gray J C
Department of Plant Sciences, University of Cambridge, UK.
Plant Mol Biol. 1992 Nov;20(3):549-54. doi: 10.1007/BF00040613.
A cDNA clone encoding the complete precursor of the delta subunit of chloroplast ATP synthase has been isolated from a tobacco (Nicotiana tabacum) leaf cDNA library in lambda gt11. The 880 bp insert encodes a polypeptide of 248 amino acid residues, of which 61 residues constitute an N-terminal presequence and 187 residues make up the mature delta subunit. Transcription and translation of the cDNA in vitro produced a protein of 29 kDa which was imported by isolated pea chloroplasts and processed to the mature 20 kDa subunit. The delta subunit precursor was processed to the mature size by a processing peptidase present in pea stromal extracts. Hybridisation of the cDNA to Southern blots of tobacco genomic DNA suggests the presence of two genes in the haploid genome.
已从烟草（Nicotiana tabacum）叶片λgt11 cDNA文库中分离出一个编码叶绿体ATP合酶δ亚基完整前体的cDNA克隆。880 bp的插入片段编码一个由248个氨基酸残基组成的多肽，其中61个残基构成N端前导序列，187个残基构成成熟的δ亚基。该cDNA在体外转录和翻译产生了一个29 kDa的蛋白质，该蛋白质被分离的豌豆叶绿体导入并加工成成熟的20 kDa亚基。δ亚基前体被豌豆基质提取物中存在的加工肽酶加工成成熟大小。该cDNA与烟草基因组DNA Southern杂交印迹表明单倍体基因组中存在两个基因。
