Salter A H, Newman B J, Napier J A, Gray J C
Department of Biochemistry, Arrhenius Laboratory for Natural Sciences, University of Stockholm, Sweden.
Plant Mol Biol. 1992 Nov;20(3):569-74. doi: 10.1007/BF00040617.
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome.
利用针对纯化的豌豆叶绿体 Rieske FeS 蛋白的抗体进行筛选后，已从 λgt 11 载体中的豌豆叶片 cDNA 文库中分离出编码叶绿体类囊体膜 Rieske FeS 蛋白前体的 cDNA 克隆。最长的 880 bp cDNA 插入片段编码一个由 230 个氨基酸残基组成的多肽，其中 50 个残基构成 N 端可裂解的前导序列，180 个残基组成成熟蛋白。该 cDNA 在体外的转录和翻译产生了一个 26 kDa 的多肽，它能被分离的豌豆叶绿体有效导入并加工成成熟的 20 kDa 蛋白。与豌豆基因组 DNA 的 Southern 杂交表明，单倍体基因组中存在一个编码 Rieske FeS 蛋白的单一基因。
