DIAS F F, BHAT J V
Appl Microbiol. 1964 Sep;12(5):412-7. doi: 10.1128/am.12.5.412-417.1964.
Over 300 bacterial strains were isolated from seven samples of activated sludge by plating on sewage agar. Gram-negative bacteria of the genera Zoogloea and Comamonas predominated. Many isolates (51%) showed sudanophilic inclusions of poly-beta-hydroxybutyric acid, whereas 34% accumulated iodophilic material on media containing starch. A large number required either vitamins or amino acids, or both, for growth. None of the isolates tested for their ability to bring about changes in autoclaved sewage produced an effluent comparable in quality to the activated sludge control, although the Zoogloea did produce activated sludgelike flocs. A study of 150 bacterial strains isolated from raw sewage revealed that they differed from the sludge isolates in several respects. Coliforms, which constitute nearly a quarter of the sewage isolates, were rarely encountered in sludge.
通过在污水琼脂平板上培养，从7份活性污泥样品中分离出300多种细菌菌株。动胶菌属和丛毛单胞菌属的革兰氏阴性菌占主导地位。许多分离株（51%）显示出嗜苏丹性的聚-β-羟基丁酸内含物，而34%的分离株在含有淀粉的培养基上积累嗜碘物质。大量菌株生长需要维生素或氨基酸，或两者都需要。尽管动胶菌确实产生了活性污泥样絮体，但对所测试的分离株改变灭菌污水的能力进行研究时，没有一种分离株产生的流出物质量能与活性污泥对照相媲美。对从原污水中分离出的150种细菌菌株的研究表明，它们在几个方面与污泥分离株不同。占污水分离株近四分之一的大肠菌群在污泥中很少见。
