AMIEL J L, MATHE G, MATSUKURA M, MERY A M, DAGUET G, TENENBAUM R, GARATTINI S, BREZIN C, PALMA V
Immunology. 1964 Sep;7(5):511-26.
The differences reported by various authors in regard to the inhibitory effect of the same antimitotic product on the production of antibodies, may be attributable in particular to the antigen used and to the technique of administration of the product. These differences have made it necessary to work out a test that is not subject to criticism in these respects. After an investigation of the cytological reactions provoked in the mouse by various antigens, the authors have singled out three of these antigens: human albumin, the poliomyelitis virus and an allogeneic skin graft. The hyperbasophilic cells which are predominantly present in the lymphoid centres are, respectively, lymphocytes for the first, plasmocytes for the second and histiocytes for the third of these antigens. In view of the different mechanisms of action that are possible with the different antimitotic products the authors used three methods of administration: entirely before' the antigenic stimulus; total dose administered entirely after' the antigenic stimulus; total dose administered partially before' and partially after' the antigenic stimulus.
不同作者报告的关于同一抗有丝分裂产物对抗体产生的抑制作用的差异，可能尤其归因于所使用的抗原以及该产物的给药技术。这些差异使得有必要设计出一种在这些方面不受批评的试验。在对各种抗原在小鼠体内引发的细胞学反应进行研究之后，作者们挑选出了其中三种抗原：人白蛋白、脊髓灰质炎病毒和同种异体皮肤移植。在这些抗原中，主要存在于淋巴中心的嗜碱性增强细胞，第一种抗原对应的是淋巴细胞，第二种是浆细胞，第三种是组织细胞。鉴于不同抗有丝分裂产物可能存在不同的作用机制，作者们采用了三种给药方法：完全在抗原刺激“之前”；总剂量完全在抗原刺激“之后”给药；总剂量部分在抗原刺激“之前”给药，部分在抗原刺激“之后”给药。
