ANACKER R L, FUKUSHI K, PICKENS E G, LACKMAN D B
J Bacteriol. 1964 Oct;88(4):1130-8. doi: 10.1128/jb.88.4.1130-1138.1964.
Anacker, R. L. (Rocky Mountain Laboratory, Hamilton, Mont.), K. Fukushi, E. G. Pickens, and D. B. Lackman. Electron microscopic observations of the development of Coxiella burnetii in the chick yolk sac. J. Bacteriol. 88:1130-1138. 1964.-Yolk sac material, obtained daily over a period of 1 week from embryos inoculated with seed of phase I Coxiella burnetii strain Ohio 314 containing 250 units of penicillin, was examined by electron microscopy and other techniques for the presence of rickettsiae. The concentration of rickettsiae in the yolk sac, as determined by electron microscopy, light microscopy, the complement-fixation test, recovery of organisms, and mouse infectivity, was low for the first 3 days, increased rapidly 3 to 5 days after infection, and then remained relatively constant. Rickettsiae in 3- to 7-day cultures, when observed by electron microscopy, had dense fibrillar centers surrounded by less-dense cytoplasmic material containing granules approximately 15 mmu in diameter. The whole was enclosed by multiple external layers. Many appeared to be in various stages of binary fission, and one form which contained a cross-wall was observed. These forms readily combined with ferritin-labeled specific antibody. In rare instances, several kinds of "atypical" forms which did not combine with ferritin-labeled antibody were found in the cytoplasm of yolk-sac cells 4 to 5 days after inoculation; it is not certain whether these forms are artifacts or normal stages in the maturation of C. burnetii. These atypical forms were not observed in subsequent experiments in which embryonated eggs were inoculated with doses of penicillin varying from 0 to 4,000 units per egg.
阿纳克，R. L.（蒙大拿州汉密尔顿落基山实验室），K. 福久志、E. G. 皮肯斯和D. B. 拉克曼。鸡卵黄囊中伯氏考克斯氏体发育的电子显微镜观察。《细菌学杂志》88:1130 - 1138。1964年。——从接种了含有250单位青霉素的I相伯氏考克斯氏体菌株俄亥俄314种子的胚胎中，在1周的时间里每天获取卵黄囊材料，通过电子显微镜和其他技术检查立克次氏体的存在情况。通过电子显微镜、光学显微镜、补体结合试验、生物体回收和小鼠感染性测定，卵黄囊中立克次氏体的浓度在前3天较低，感染后3至5天迅速增加，然后保持相对稳定。在3至7天培养物中的立克次氏体，通过电子显微镜观察，有致密的纤维状中心，周围是密度较低的细胞质物质，含有直径约15毫微米的颗粒。整体被多层外层包围。许多似乎处于二分裂的不同阶段，并且观察到一种含有横壁的形态。这些形态很容易与铁蛋白标记的特异性抗体结合。在罕见的情况下，接种后4至5天在卵黄囊细胞的细胞质中发现了几种不与铁蛋白标记抗体结合的“非典型”形态；不确定这些形态是人为产物还是伯氏考克斯氏体成熟过程中的正常阶段。在随后用每枚鸡蛋0至4000单位不同剂量青霉素接种胚胎卵的实验中未观察到这些非典型形态。
