ROGERS C G, SARLES W B
J Bacteriol. 1964 Oct;88(4):965-73. doi: 10.1128/jb.88.4.965-973.1964.
Rogers, C. G. (Department of National Health and Welfare, Ottawa, Ontario, Canada), and W. B. Sarles. Isolation and identification of enterococci from the intestinal tract of the rat. J. Bacteriol. 88:965-973. 1964.-Surface inoculation was employed in a comparison of three selective media, M-enterococcus (M) agar, thallium acetate-glucose (TITG) agar, and KF-streptococcus (KF) agar, for enumeration and isolation of enterococci from contents of the digestive tract of the rat. Similar yields of enterococcus colonies were obtained with M, TITG, and KF agar. When cecal contents were examined, species identification of 120 isolates, 40 from each medium, revealed only minor differences in selective activity. Streptococcus faecalis and its variety zymogenes represented 55 to 63% of the isolates from each medium; the remaining strains resembled S. durans or S. faecium. More than 99% of the isolates failed to survive the heat-tellurite tolerance test. Periodic analyses of intestinal contents from young rats indicated that enterococci became established in the gut from 10 to 14 days after birth. The species isolated most frequently on M agar was S. faecalis var. zymogenes. When rats were fed a purified diet with casein as the source of nitrogen, S. faecalis var. zymogenes predominated among isolates from contents of the small intestine plated on M agar. This species was largely replaced by others resembling S. durans or S. faecium when mixtures of l-amino acids provided the dietary nitrogen. Nineteen enterococcus isolates, representing all that met the Sherman criteria, required arginine, glutamic acid, histidine, isoleucine, leucine, methionine, tryptophan, and valine; of these, two strains required in addition threonine, glycine, and lysine; four required threonine and glycine; two, threonine and lysine; and one, threonine.
罗杰斯，C.G.（加拿大安大略省渥太华国家卫生与福利部）以及W.B.萨尔斯。从大鼠肠道分离和鉴定肠球菌。《细菌学杂志》88:965 - 973。1964年。——采用表面接种法比较了三种选择性培养基，即M - 肠球菌（M）琼脂、醋酸铊 - 葡萄糖（TITG）琼脂和KF - 链球菌（KF）琼脂，用于从大鼠消化道内容物中计数和分离肠球菌。用M、TITG和KF琼脂获得的肠球菌菌落产量相似。检查盲肠内容物时，对从每种培养基分离出的120株菌株（每种培养基40株）进行菌种鉴定，结果显示选择性活性仅有微小差异。粪链球菌及其发酵变种占每种培养基分离菌株的55%至63%；其余菌株类似耐久链球菌或屎肠球菌。超过99%的分离菌株在耐热碲酸盐耐受性试验中未能存活。对幼鼠肠道内容物的定期分析表明，肠球菌在出生后10至14天内在肠道中定植。在M琼脂上最常分离出的菌种是粪链球菌发酵变种。当给大鼠喂食以酪蛋白为氮源的纯化日粮时，在接种于M琼脂的小肠内容物分离菌株中，粪链球菌发酵变种占主导。当以L - 氨基酸混合物作为日粮氮源时，该菌种在很大程度上被类似耐久链球菌或屎肠球菌的其他菌种所取代。19株符合谢尔曼标准的肠球菌分离菌株需要精氨酸、谷氨酸、组氨酸、异亮氨酸、亮氨酸、蛋氨酸、色氨酸和缬氨酸；其中，两株菌株还需要苏氨酸、甘氨酸和赖氨酸；四株需要苏氨酸和甘氨酸；两株需要苏氨酸和赖氨酸；一株需要苏氨酸。
