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使用头孢氨苄-氨曲南-阿拉伯糖琼脂选择性分离屎肠球菌。

Use of cephalexin-aztreonam-arabinose agar for selective isolation of Enterococcus faecium.

作者信息

Ford M, Perry J D, Gould F K

机构信息

Microbiology Department, Freeman Hospital, High Heaton, Newcastle upon Tyne, United Kingdom.

出版信息

J Clin Microbiol. 1994 Dec;32(12):2999-3001. doi: 10.1128/jcm.32.12.2999-3001.1994.

DOI:10.1128/jcm.32.12.2999-3001.1994
PMID:7883889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264214/
Abstract

Cephalexin-aztreonam-arabinose agar (CAA), a new selective agar, was examined in comparison with nalidixic acid-colistin agar for the differentiation of Enterococcus faecium from other enterococci and the ability to isolate the organism from feces. Two hundred sixteen enterococcus isolates and a variety of gram-positive and gram-negative control strains were inoculated onto both media. All control strains of E. faecium were easily differentiated from Enterococcus faecalis and Enterococcus durans on the basis of arabinose fermentation on CAA. Differentiation of E. faecium from other enterococci or Streptococcus bovis was not possible on nalidixic acid-colistin agar. Increased isolation of E. faecium was demonstrated on CAA when both media were compared for the isolation of the organism from feces. CAA has been shown to possess excellent differential and selective features allowing the simple and effective isolation of E. faecium from heavily contaminated sites.

摘要

头孢氨苄-氨曲南-阿拉伯糖琼脂(CAA)是一种新型选择性琼脂,将其与萘啶酸-黏菌素琼脂进行比较,以研究其在区分屎肠球菌与其他肠球菌方面的作用以及从粪便中分离该菌的能力。将216株肠球菌分离株以及多种革兰氏阳性和革兰氏阴性对照菌株接种到两种培养基上。基于在CAA上的阿拉伯糖发酵情况,所有屎肠球菌对照菌株都能轻易与粪肠球菌和耐久肠球菌区分开来。在萘啶酸-黏菌素琼脂上无法区分屎肠球菌与其他肠球菌或牛链球菌。当比较两种培养基从粪便中分离该菌的能力时,CAA上屎肠球菌的分离率有所提高。已证明CAA具有出色的鉴别和选择特性,能够从严重污染的样本中简单有效地分离出屎肠球菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6397/264214/3f23aad7b46b/jcm00012-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6397/264214/3f23aad7b46b/jcm00012-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6397/264214/3f23aad7b46b/jcm00012-0130-a.jpg

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