两种蓝藻中的C-藻蓝蛋白和别藻蓝蛋白。
C-phycocyanin and allophycocyanin in two species of blue-green algae.
作者信息
Craig I W, Carr N G
出版信息
Biochem J. 1968 Jan;106(2):361-6. doi: 10.1042/bj1060361.
Abstract
- The biliproteins C-phycocyanin and allophycocyanin were purified from the blue-green alga Anabaena variabilis by ammonium sulphate fractionation and gel filtration. 2. An assay procedure that enabled the proportion of the two pigments, present as a mixture, to be determined was devised by using the data provided by spectrophotometric analysis of the purified biliproteins. 3. The degree of association and relative proportions of the two pigments were analysed by the application of this procedure to the components separated by thin-layer gel filtration. 4. The C-phycocyanin/allophycocyanin ratio remained essentially constant in algal extracts prepared at various stages throughout the growth cycle or after growth under conditions of reduced illumination. 5. The behaviour of the C-phycocyanin aggregate species from Anacystis nidulans suggested that they were of appreciably lower molecular weight than those observed in extracts of Anabaena variabilis.
摘要
- 通过硫酸铵分级分离和凝胶过滤，从蓝藻可变鱼腥藻中纯化出了藻胆蛋白C-藻蓝蛋白和别藻蓝蛋白。2. 利用对纯化后的藻胆蛋白进行分光光度分析所提供的数据，设计了一种测定方法，该方法能够确定以混合物形式存在的两种色素的比例。3. 通过将该方法应用于经薄层凝胶过滤分离的组分，分析了两种色素的缔合程度和相对比例。4. 在整个生长周期的不同阶段制备的藻类提取物中，或在光照减弱条件下生长后的藻类提取物中，C-藻蓝蛋白/别藻蓝蛋白的比例基本保持恒定。5. 来自集胞藻的C-藻蓝蛋白聚集体物种的行为表明，它们的分子量明显低于在可变鱼腥藻提取物中观察到的分子量。
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