枯草芽孢杆菌产生细胞外5'-鸟苷酸
PRODUCTION OF EXTRACELLULAR GUANOSINE-5'-MONOPHOSPHATE BY BACILLUS SUBTILIS.
作者信息
DEMAIN A L, MILLER I M, HENDLIN D
出版信息
J Bacteriol. 1964 Oct;88(4):991-5. doi: 10.1128/jb.88.4.991-995.1964.
Demain, A. L. (Merck Sharp & Dohme Research Laboratories, Rahway, N.J.), I. M. Miller, and D. Hendlin. Production of extracellular guanosine-5'-monophosphate by Bacillus subtilis. J. Bacteriol. 88:991-995. 1964.-Wild-type Bacillus subtilis colonies were found to feed purineless mutants. A strain with high feeding capacity was selected for study, with a guanineless mutant of B. subtilis used as the assay organism. The factor was excreted during its growth phase in a complex medium containing starch and soybean meal extract. Nutritional studies led to the development of a defined medium to be used for biochemical studies and to aid in the isolation of the factor. Starch was replaced by maltose and the soybean meal extract by Mn(++). Production of the factor was sensitive to the pH of the medium during growth. Practically its entire extracellular accumulation occurred before visible lysis. The factor was identified as guanosine-5'-monophosphate derived by extracellular enzymatic hydrolysis of excreted ribonucleic acid.
德缅，A. L.（新泽西州拉威市默克雪兰诺研究实验室），I. M. 米勒，以及D. 亨德林。枯草芽孢杆菌产生细胞外5'-磷酸鸟苷。《细菌学杂志》88:991 - 995。1964年。发现野生型枯草芽孢杆菌菌落能为无嘌呤突变体提供营养。选择了一株具有高营养供给能力的菌株进行研究，以枯草芽孢杆菌的无鸟嘌呤突变体作为检测生物体。该因子在含有淀粉和大豆粉提取物的复合培养基中生长阶段分泌。营养研究促成了一种用于生化研究并有助于该因子分离的限定培养基的开发。用麦芽糖替代淀粉，用Mn(++)替代大豆粉提取物。该因子的产生在生长期间对培养基的pH敏感。实际上其细胞外的全部积累在可见裂解之前就已发生。该因子被鉴定为通过对分泌的核糖核酸进行细胞外酶促水解而产生的5'-磷酸鸟苷。
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