枯草芽孢杆菌对核糖核酸的排泄与降解
EXCRETION AND DEGRADATION OF RIBONUCLEIC ACID BY BACILLUS SUBTILIS.
作者信息
DEMAIN A L, BURG R W, HENDLIN D
出版信息
J Bacteriol. 1965 Mar;89(3):640-6. doi: 10.1128/jb.89.3.640-646.1965.
Demain, A. L. (Merck & Co., Inc., Rahway, N.J.), R. W. Burg, and D. Hendlin. Excretion and degradation of ribonucleic acid by Bacillus subtilis. J. Bacteriol. 89:640-646. 1965.-Bacillus subtilis MB-1480 was found to produce several 5'-ribonucleotides in the extracellular medium, in addition to the previously found 5'-guanosine monophosphate and guanosine diphosphate. The nucleotides accumulated after excretion of high-molecular-weight ribonucleic acid (RNA) and subsequent extracellular breakdown. Excretion of RNA paralleled growth closely, and was accompanied by deoxyribonucleic acid (DNA) and protein excretion. The process occurred in the absence of visible lysis. Whereas extracellular DNA was stable, the other macromolecules were degraded after excretion. With extracellular DNA as a measure of maximal lysis, it was calculated that the major part of the extracellular RNA must have been excreted by cells which had not undergone lysis.
德梅因，A. L.（默克公司，新泽西州拉威市）、R. W. 伯格和D. 亨德林。枯草芽孢杆菌对核糖核酸的排泄与降解。《细菌学杂志》89:640 - 646。1965年。——发现枯草芽孢杆菌MB - 1480除了先前发现的5'-鸟苷单磷酸和鸟苷二磷酸外，还能在细胞外培养基中产生几种5'-核糖核苷酸。这些核苷酸在高分子量核糖核酸（RNA）排泄及随后的细胞外分解后积累。RNA的排泄与生长密切平行，并伴有脱氧核糖核酸（DNA）和蛋白质的排泄。该过程在无明显裂解的情况下发生。细胞外DNA稳定，而其他大分子在排泄后被降解。以细胞外DNA作为最大裂解的衡量标准，计算得出细胞外RNA的主要部分必定是由未发生裂解的细胞排泄的。
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