Gu Yian, Zeleniuch-Jacquotte Anne, Linkov Faina, Koenig Karen L, Liu Mengling, Velikokhatnaya Lyudmila, Shore Roy E, Marrangoni Adele, Toniolo Paolo, Lokshin Anna E, Arslan Alan A
Department of Environmental Medicine, New York University School of Medicine, 650 First Avenue, New York, NY 10016, USA.
Cytokine. 2009 Jan;45(1):44-9. doi: 10.1016/j.cyto.2008.10.014. Epub 2008 Dec 5.
In most studies, circulating biomarkers are usually assessed from a single sample, assuming that this single measurement represents the long-term biomarker status of the individual. Such an assumption is rarely tested although it may not be valid for all biomarkers. The objective of this study was to investigate the temporal reproducibility of a panel of cytokines and growth factors.
Thirty-five postmenopausal women with two annual visits and 30 premenopausal women with three annual visits were randomly selected from the participants in an existing prospective cohort. A total of 23 serum cytokines, nine growth factors and C-reactive protein (CRP) were measured using the Luminex xMap technology. In addition, for eight biomarkers, regular and high sensitivity (hs) assays were compared.
The biomarkers with adequate (>60%) detection rates and acceptable (> or =0.55) intra-class correlation coefficients (ICCs) were: hsIL-1beta, IL-1RA, hsIL-2, hsIL-4, hsIL-5, hsIL-6, hsIL-10, IL-12p40, hsIL-12p70, hsTNF-alpha, TNF-R1, TNF-R2, CRP, HGF, NGF, and EGFR. The remaining biomarkers either had low temporal reproducibility or were undetectable in more than 40% of samples.
The results suggest that 16 of the 41 biomarkers measured with Luminex technology showed sufficient sensitivity and temporal reproducibility in sera.
在大多数研究中，循环生物标志物通常从单个样本中进行评估，假定这一单次测量代表个体的长期生物标志物状态。尽管这种假设可能并非对所有生物标志物都有效，但很少有人对此进行检验。本研究的目的是调查一组细胞因子和生长因子的时间可重复性。
从现有的前瞻性队列研究参与者中随机选取35名绝经后女性（每年随访两次）和30名绝经前女性（每年随访三次）。使用Luminex xMap技术检测了总共23种血清细胞因子、9种生长因子和C反应蛋白（CRP）。此外，对8种生物标志物比较了常规检测和高灵敏度（hs）检测。
检测率足够高（>60%）且组内相关系数（ICC）可接受（>或=0.55）的生物标志物有：hsIL-1β、IL-1RA、hsIL-2、hsIL-4、hsIL-5、hsIL-6、hsIL-10、IL-12p40、hsIL-12p70、hsTNF-α、TNF-R1、TNF-R2、CRP、HGF、NGF和EGFR。其余生物标志物要么时间可重复性低，要么在超过40%的样本中无法检测到。
结果表明，用Luminex技术检测的41种生物标志物中有16种在血清中显示出足够的灵敏度和时间可重复性。
