Morgunov A A, Bulgakov V G, Bilenko M V
Eksp Klin Farmakol. 1992 Mar-Apr;55(2):17-20.
The anti-ischemic and toxic effects of different doses of the antioxidant ionol (butyl hydroxytoluene, BHT) introduced into the Krebs-Henseleit medium composed of monolayer liposomes of egg phospholipids before or 30 minutes after total ischemia of the heart were studied on a model of perfusion of isolated Wistar rat heart by the method of Langendorff-Fallen. It has been demonstrated that ionol, after its addition to the perfusate in the preischemic period, exerts an anti-ischemic effect in concentrations of 10(-6), 3 x 10(-6) and 10(-5) M; in the postischemic period, in concentrations of 3 x 10(-6) and 10(-5); the protective effect of ionol in the postischemic period is less pronounced. In higher doses (3 x 10(-5) and 10(-4) M) ionol produces a toxic action which is more remarkable and is less reversible in respect to the ischemized than to the nonischemized heart, and is realized rather through the dysfunction of heart muscle relaxation. The conclusion is drawn that there is a possibility of using ionol in doses of 10(-6) to 10(-5) M as a constituent of liposomes for addition to perfusion, conservant and cardioplegic solutions as an anti-ischemic remedy.
采用Langendorff-Fallen法，在离体Wistar大鼠心脏灌注模型上，研究了在心脏完全缺血前或缺血30分钟后，将不同剂量的抗氧化剂离子醇（丁基羟基甲苯，BHT）加入由卵磷脂单层脂质体组成的Krebs-Henseleit培养基中时的抗缺血和毒性作用。结果表明，在缺血前期将离子醇加入灌注液中时，其在浓度为10^(-6)、3×10^(-6)和10^(-5) M时发挥抗缺血作用；在缺血后期，浓度为3×10^(-6)和10^(-5)时发挥作用；离子醇在缺血后期的保护作用不太明显。在较高剂量（3×10^(-5)和10^(-4) M）时，离子醇产生毒性作用，这种作用在缺血心脏中比在未缺血心脏中更显著且更难逆转，并且主要通过心肌舒张功能障碍来实现。得出的结论是，有可能将剂量为10^(-6)至10^(-5) M的离子醇作为脂质体的成分，添加到灌注液、保存液和心脏停搏液中，作为一种抗缺血药物。
