Owens W, Field J K, Howard P J, Stell P M
University of Liverpool.
Eur J Cancer B Oral Oncol. 1992 Jul;28B(1):17-21. doi: 10.1016/0964-1955(92)90006-m.
Chromosomal abnormalities in short term cultures have been investigated in 10 squamous cell carcinomas of the head and neck. Of these tumours, three demonstrated clonal chromosomal abnormalities, two showed random abnormalities and 5 patients' tumours had normal karyotypes. The 5 patients with aberrant karyotypes were all from previously treated tumours, of these, 4 patients had received radiotherapy and 1 surgery. On analysis of the three clonal tumours, two were found to be polyclonal, each with five separate clones. 116 breakpoints were demonstrated from the clonal data of these tumours, and all of the chromosomes were involved, apart from number 18. In this study we found three or more breakpoints at sites 1p36, 9q32 and 11q23. 1 of the patients investigated showed a clonal abnormality involving a breakpoint at the 11q13 site, with a further 2 patients having breakpoints at 1p22--sites previously reported to have marked clustering of cytogenetic abnormalities in oral cancer patients. Only further studies will demonstrate whether the breakpoints found are of clinical significance.
对10例头颈部鳞状细胞癌的短期培养物中的染色体异常进行了研究。在这些肿瘤中，3例显示出克隆性染色体异常，2例显示随机异常，5例患者的肿瘤具有正常核型。5例核型异常的患者均来自先前接受过治疗的肿瘤，其中4例接受了放疗，1例接受了手术。对3例克隆性肿瘤进行分析时，发现其中2例为多克隆性，各有5个独立的克隆。从这些肿瘤的克隆数据中显示出116个断点，除18号染色体外，所有染色体均有涉及。在本研究中，我们发现在1p36、9q32和11q23位点有三个或更多断点。所研究的1例患者显示出涉及11q13位点断点的克隆性异常，另外2例患者在1p22位点有断点——此前有报道称该位点在口腔癌患者中存在明显的细胞遗传学异常聚集。只有进一步的研究才能证明所发现的断点是否具有临床意义。
