Hermann K, von Eschenbach C E, von Tschirschnitz M, Ring J
Department of Dermatology, University Hospital Eppendorf, University of Hamburg, Germany.
Regul Pept. 1993 Nov 19;49(1):1-7. doi: 10.1016/0167-0115(93)90377-k.
The plasma concentrations of arginine vasopressin, oxytocin, angiotensin I and II were studied in patients with hymenoptera venom anaphylaxis (n = 50) and healthy volunteers (n = 25). There was no difference in arginine vasopressin: 5.52 +/- 0.45 fmol/ml vs. 3.99 +/- 0.41 fmol/ml or oxytocin: 28.10 +/- 1.13 fmol/ml vs. 26.24 +/- 1.80 fmol/ml between patients and controls. No correlation between the severity of clinical symptoms and the plasma levels of arginine vasopressin and oxytocin was found in patients. However, patients with a history of hymenoptera venom anaphylaxis showed significantly reduced angiotensin I and angiotensin II plasma levels as compared to controls (ANG I: 9.51 +/- 0.61 fmol/ml vs. 22.91 +/- 1.73 fmol/ml; ANG II: 2.84 +/- 0.16 fmol/ml vs. 6.95 +/- 0.33 fmol/ml). A significant inverse correlation between the severity of clinical symptoms and the plasma levels of angiotensin I and angiotensin II was observed; the lower the concentrations the more severe the clinical symptoms. Oxytocin immunoreactivity eluted from the HPLC column as a single peak with the same retention time as synthetic oxytocin. The vasopressin immunoreactive material could be characterized on HPLC as arginine-vasopressin and two other peptides of unknown nature which crossreacted with the vasopressin antibody. These findings suggest a possible role of angiotensin I and angiotensin II in hymenoptera venom anaphylaxis while arginine vasopressin and oxytocin are most likely not involved.
对50例膜翅目毒液过敏患者和25名健康志愿者的精氨酸加压素、催产素、血管紧张素I和血管紧张素II的血浆浓度进行了研究。患者和对照组之间的精氨酸加压素（分别为5.52±0.45 fmol/ml和3.99±0.41 fmol/ml）或催产素（分别为28.10±1.13 fmol/ml和26.24±1.80 fmol/ml）无差异。在患者中，未发现临床症状严重程度与精氨酸加压素和催产素血浆水平之间存在相关性。然而，与对照组相比，有膜翅目毒液过敏史的患者血管紧张素I和血管紧张素II的血浆水平显著降低（血管紧张素I：9.51±0.61 fmol/ml vs. 22.91±1.73 fmol/ml；血管紧张素II：2.84±0.16 fmol/ml vs. 6.95±0.33 fmol/ml）。观察到临床症状严重程度与血管紧张素I和血管紧张素II血浆水平之间存在显著负相关；浓度越低，临床症状越严重。从HPLC柱上洗脱的催产素免疫反应性以单峰形式出现，保留时间与合成催产素相同。加压素免疫反应性物质在HPLC上可鉴定为精氨酸加压素和另外两种性质未知的与加压素抗体发生交叉反应的肽。这些发现表明血管紧张素I和血管紧张素II在膜翅目毒液过敏中可能起作用，而精氨酸加压素和催产素很可能不参与其中。
