Mandai T, Yoneyama M, Sakai S, Muto N, Yamamoto I
Amase Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
Carbohydr Res. 1992 Aug 3;232(2):197-205. doi: 10.1016/0008-6215(92)80054-5.
The stable L-ascorbic acid glucoside produced by the action of the cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) from Bacillus stearothermophilus was crystallized from an aqueous solution. Determination of the molecular structure by single crystal X-ray analysis showed the compound to be 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G). The crystals are orthorhombic, space group P2(1)2(1)2(1), with unit-cell dimensions a = 11.929 A, b = 24.351 A, and c = 4.864 A. The D-glucopyranose residue has the 4C1 conformation. These conclusions are in good agreement with those based on the 13C-NMR spectrum. The general physicochemical properties of crystalline AA-2G are reported.
嗜热脂肪芽孢杆菌的环麦芽糊精葡糖基转移酶（CGTase，EC 2.4.1.19）作用产生的稳定的L-抗坏血酸葡糖苷从水溶液中结晶出来。通过单晶X射线分析确定分子结构，结果表明该化合物为2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G）。晶体为正交晶系，空间群P2(1)2(1)2(1)，晶胞参数a = 11.929 Å，b = 24.351 Å，c = 4.864 Å。D-吡喃葡萄糖残基具有4C1构象。这些结论与基于13C-NMR光谱得出的结论高度一致。本文报道了结晶AA-2G的一般物理化学性质。
