Lexell J, Jarvis J, Downham D, Salmons S
Department of Human Anatomy and Cell Biology, University of Liverpool, UK.
Cell Tissue Res. 1992 Aug;269(2):195-204. doi: 10.1007/BF00319609.
The purpose of this study was to examine the contention that stimulation-induced damage, resulting in degeneration with subsequent regeneration, plays a major role in the transformation of fibre type brought about by chronic electrical stimulation. Data from histological and histochemical sections of 9-day-stimulated rabbit fast-twitch muscles were analysed with multivariate statistical techniques. Fibre degeneration and regeneration varied non-systematically between sample areas at any given cross-sectional level. In the extensor digitorum longus muscle, but not in the tibialis anterior, there was more degeneration in proximal than in distal portions of the muscle. The extensor digitorum longus muscle consistently showed more degeneration than the tibialis anterior muscle. Degeneration was less extensive for an intermittent pattern of stimulation that delivered half the aggregate number of impulses of continuous stimulation. Degeneration and regeneration varied markedly between individual rabbits in each of the groups. Sections that revealed the most degeneration and regeneration also had more fibres that reacted positively with an anti-neonatal antibody. Rigorous analysis of different sources of variation has helped to explain apparent conflicts in the literature. The incidence of muscle fibre damage in the stimulated tibialis anterior muscle is low, showing that the contribution of degenerative-regenerative phenomena to fibre type conversion in this muscle is insignificant.
本研究的目的是检验一种观点，即刺激诱导的损伤导致退变并随后再生，在慢性电刺激引起的纤维类型转变中起主要作用。运用多元统计技术分析了对家兔快肌进行9天刺激后的组织学和组织化学切片数据。在任何给定的横截面水平上，样本区域之间的纤维退变和再生并无系统差异。在趾长伸肌中，而非胫骨前肌，肌肉近端的退变比远端更严重。趾长伸肌的退变始终比胫骨前肌更广泛。对于间歇性刺激模式，其传递的总脉冲数为连续刺激的一半，退变程度较轻。每组中的各个家兔之间，退变和再生情况差异显著。显示出最严重退变和再生的切片中，也有更多与抗新生儿抗体呈阳性反应的纤维。对不同变异来源的严格分析有助于解释文献中明显的矛盾之处。受刺激的胫骨前肌中肌纤维损伤的发生率较低，表明退变 - 再生现象对该肌肉纤维类型转换的贡献微不足道。
