Pieri C, Moroni F, Recchioni R
Cytology Center, Gerontology Research Department of I.N.R.C.A., Ancona, Italy.
Cell Immunol. 1992 Nov;145(1):210-7. doi: 10.1016/0008-8749(92)90324-i.
The time-dependent changes of mitochondrial membrane potential and mass have been investigated on rat splenic lymphocytes stimulated with Con A in the presence and absence of reduced glutathione (GSH). Rhodamine-123 (Rh-123) and nonyl acridine orange (NAO) were used as specific dyes to monitor the membrane potential and mass of mitochondria, respectively. The percentage of cells showing blast transformation and the level of Rh-123 or NAO uptake were analyzed by flow cytometry. Present results demonstrate that a large number of cells showed activated mitochondria already at 24 hr after Con A stimulation and the activation of these organelles was not related to blast transformation. The addition of GSH into the culture medium increased the number of cells responding to mitogenic stimulation. In parallel it augmented the percentage of lymphocytes with activated mitochondria and also prevented their depolarization.
在有和没有还原型谷胱甘肽（GSH）存在的情况下，研究了用刀豆蛋白A（Con A）刺激的大鼠脾淋巴细胞中线粒体膜电位和质量随时间的变化。分别使用罗丹明-123（Rh-123）和壬基吖啶橙（NAO）作为特异性染料来监测线粒体的膜电位和质量。通过流式细胞术分析显示有母细胞转化的细胞百分比以及Rh-123或NAO摄取水平。目前的结果表明，大量细胞在Con A刺激后24小时就已经显示出线粒体被激活，并且这些细胞器的激活与母细胞转化无关。向培养基中添加GSH增加了对有丝分裂原刺激作出反应的细胞数量。同时，它增加了线粒体被激活的淋巴细胞百分比，并且还防止了它们的去极化。
