白喉毒素作用机制的研究。I. 正常及中毒的海拉细胞中的磷酸化中间体
STUDIES ON THE MODE OF ACTION OF DIPHTHERIA TOXIN. I. PHOSPHORYLATED INTERMEDIATES IN NORMAL AND INTOXICATED HELA CELLS.
作者信息
COLLIER R J, PAPPENHEIMER A M
出版信息
J Exp Med. 1964 Dec 1;120(6):1007-18. doi: 10.1084/jem.120.6.1007.
Abstract
Intracellular levels of ATP, GTP, and hexose phosphates have been determined in HeLa cells at intervals after exposure to saturating doses of diphtheria toxin. Toxin causes no significant change in the level of any of these phosphorylated intermediates either in the presence or absence of glucose over a period of at least 5 to 6 hours. It is concluded that the inhibition of protein synthesis which occurs in HeLa cells at about 2 hours after the addition of saturating doses of toxin, does not result from an effect of toxin on energy metabolism.
摘要
在用饱和剂量的白喉毒素处理后，每隔一段时间测定了HeLa细胞内ATP、GTP和己糖磷酸的水平。在至少5至6小时的时间段内，无论有无葡萄糖存在，毒素对这些磷酸化中间体的水平均无显著影响。得出的结论是，在加入饱和剂量毒素后约2小时，HeLa细胞中发生的蛋白质合成抑制并非毒素对能量代谢作用的结果。
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