Goor R S, Pappenheimer A M
J Exp Med. 1967 Nov 1;126(5):899-912. doi: 10.1084/jem.126.5.899.
Extracts from HeLa cells treated with excess diphtheria toxin for several hours, until all protein synthesis has been arrested, are still able to stimulate the poly U-directed incorporation of phenylalanine into polypeptides at a moderate rate. Activity may be restored to normal levels or above by addition of a soluble enzyme fraction containing transferase II. Our results are in agreement with those of Collier who has recently shown that toxin inactivates transferase II in extracts from rabbit reticulocytes. We have further demonstrated that amino acid incorporation in extracts from intoxicated HeLa cells is limited by their transferase II content whereas, in extracts from normal cells, it is the ribosomes and to a lesser extent sRNA that are limiting. We have found that only soluble transferase II is inactivated by toxin; the ribosome-bound enzyme is resistant.
用过量白喉毒素处理HeLa细胞数小时，直至所有蛋白质合成均被阻断，其提取物仍能够以一定速率刺激苯丙氨酸在多聚尿苷酸引导下掺入多肽。通过添加含有转移酶II的可溶性酶组分，活性可恢复至正常水平或更高。我们的结果与科利尔的结果一致，他最近表明毒素可使兔网织红细胞提取物中的转移酶II失活。我们进一步证明，中毒的HeLa细胞提取物中的氨基酸掺入受其转移酶II含量的限制，而在正常细胞提取物中，限制因素是核糖体以及程度较轻的可溶性RNA。我们发现，只有可溶性转移酶II会被毒素失活；核糖体结合的酶具有抗性。
