COLLIER R J, PAPPENHEIMER A M
J Exp Med. 1964 Dec 1;120(6):1019-39. doi: 10.1084/jem.120.6.1019.
It has been demonstrated that low concentrations of highly purified diphtheria toxin specifically inhibit incorporation of labeled amino acids into polypeptides in extracts from HeLa cells and from rabbit reticulocytes. No inhibition of incorporation occurs in the absence of a specific cofactor. This cofactor has been identified as nicotinamide adenine dinucleotide (NAD). It has been shown that it is one of the steps involving transfer of amino acids from soluble ribonucleic acid to the growing polypeptide chain that is affected by the toxin in the presence of NAD.
已经证明，低浓度的高度纯化的白喉毒素能特异性抑制标记氨基酸掺入来自HeLa细胞和兔网织红细胞提取物中的多肽。在没有特定辅因子的情况下，掺入不会受到抑制。这种辅因子已被鉴定为烟酰胺腺嘌呤二核苷酸（NAD）。已经表明，在NAD存在下，毒素影响的是氨基酸从可溶性核糖核酸转移到正在生长的多肽链这一步骤。
