TRESSELT H B, WARD M K
Appl Microbiol. 1964 Nov;12(6):504-7. doi: 10.1128/am.12.6.504-507.1964.
A medium composed of (in g/100 ml) Tryptose broth with thiamine (Difco), 2.6; cysteine-HCl, 0.12; glucose, 1; FeSO(4), 7H(2)O, 0.005; KCl, 0.02; histidine, 0.1; tris(hydroxymethyl)aminomethane (tris) buffer, 0.3; and agar, 1; will support rapid growth of the fully virulent SCHU-S4 strain of Pasteurella tularensis. Although the test organism grew rapidly on medium from which KCl and tris buffer were omitted, these two components increased the stability of the medium upon storage at 4 C. It was necessary to (i) control carefully the relative concentration of the ferrous iron and cysteine-HCl, (ii) incubate the prepared medium overnight prior to use, and (iii) incubate the inoculated plates in an atmosphere of high relative humidity. Rapid growth of the organism was obtained also from very small inocula in the liquid form of the medium. Biochemical studies designed to elucidate the mechanisms involved in the enhancement of growth of P. tularensis in this relatively simple blood-free medium were initiated.
一种由（每100毫升中含）胰蛋白胨肉汤加硫胺素（Difco）2.6克、半胱氨酸盐酸盐0.12克、葡萄糖1克、硫酸亚铁（FeSO₄）·7H₂O 0.005克、氯化钾0.02克、组氨酸0.1克、三（羟甲基）氨基甲烷（tris）缓冲液0.3克以及琼脂1克组成的培养基，能支持土拉弗朗西斯菌强毒株SCHU - S4的快速生长。尽管测试菌株在省略了氯化钾和tris缓冲液的培养基上生长迅速，但这两种成分提高了培养基在4℃储存时的稳定性。有必要（i）仔细控制亚铁离子和半胱氨酸盐酸盐的相对浓度，（ii）在使用前将制备好的培养基过夜培养，以及（iii）在高相对湿度的气氛中培养接种的平板。在这种培养基的液体形式中，即使接种量非常小，也能实现该菌株的快速生长。已开始进行生化研究，以阐明在这种相对简单的无血培养基中土拉弗朗西斯菌生长增强所涉及的机制。
