Tärnvik A, Holm S E
Infect Immun. 1978 Jun;20(3):698-704. doi: 10.1128/iai.20.3.698-704.1978.
When purified T lymphocytes from individuals vaccinated with a viable, attenuated strain of Francisella tularensis were incubated in vitro in the presence of heat-killed bacteria or a membrane preparation of the vaccine strain, they were stimulated to form blast cells and to synthesize deoxyribonucleic acid. The blast cells had the characteristics of T cells, being devoid of surface immunoglobulin and able to form rosettes with sheep erythrocytes. The stimulation occurred only when monocytes were present. A lymphocyte preparation enriched in B lymphocytes did not respond to the heat-killed bacteria or to the membrane preparation. In a stimulated mononuclear leukocyte preparation, about 70% of the blast cells formed rosettes with sheep erythrocytes, and 10 to 20% of them had surface immunoglobulin. The results show that there is an enlarged population of specifically committed T lymphocytes after tularemia vaccination. It is suggested that the lymphocyte stimulation test measures mainly T-lymphocyte reactivity when membranes or whole bacteria of F. tularensis LVS are used as antigen, and that the stimulation of human T lymphocytes by whole bacteria or bacterial membranes is completely monocyte or macrophage dependent. The present experimental procedure may provide a model for study of antigen-induced stimulation of human lymphocytes under controlled conditions. The technique used gave a reproducible, extremely purified preparation of T lymphocytes and a preparation of monocytes especially suitable for microcultures.
当将接种了活的减毒土拉弗朗西斯菌菌株的个体的纯化T淋巴细胞，在热灭活细菌或疫苗菌株的膜制剂存在的情况下于体外培养时，它们会被刺激形成母细胞并合成脱氧核糖核酸。这些母细胞具有T细胞的特征，缺乏表面免疫球蛋白且能够与绵羊红细胞形成玫瑰花结。这种刺激仅在存在单核细胞时才会发生。富含B淋巴细胞的淋巴细胞制剂对热灭活细菌或膜制剂无反应。在受刺激的单核白细胞制剂中，约70%的母细胞与绵羊红细胞形成玫瑰花结，其中10%至20%具有表面免疫球蛋白。结果表明，兔热病疫苗接种后存在大量特异性定向的T淋巴细胞。有人提出，当使用土拉弗朗西斯菌LVS的膜或全菌作为抗原时，淋巴细胞刺激试验主要检测T淋巴细胞反应性，并且全菌或细菌膜对人T淋巴细胞的刺激完全依赖于单核细胞或巨噬细胞。目前的实验程序可为在可控条件下研究抗原诱导的人淋巴细胞刺激提供一个模型。所使用的技术给出了可重复的、极其纯化的T淋巴细胞制剂以及特别适合微培养的单核细胞制剂。
