KASATIYA S S, LIEB M
J Bacteriol. 1964 Dec;88(6):1585-9. doi: 10.1128/jb.88.6.1585-1589.1964.
Kasatiya, Shanti-Swaroop (University of Southern California, Los Angeles), and Margaret Lieb. Effect of heat shock on T(4)rII multiplication in Escherichia coli. J. Bacteriol. 88:1585-1589. 1964.-Heating certain strains of Escherichia coli K-12 (lambda) to 45 to 48 C for 12 min before phage infection increased the fraction of bacteria that allowed multiplication of T(4)rII mutants. The effect of heat on interference to T(4)rII was not correlated with induction of the lambda prophage, which occurred in some of the strains studied. Incubation of heated cells at 37 C before infection resulted in the recovery of interference, providing a prophage was still present. The presence of magnesium ion after infection is known to greatly decrease the interference in K-12 (lambda) to T(4)rII. When heat-shocked cells were infected with T(4)rII, and then incubated in medium containing a suboptimal concentration of magnesium ion, more cells produced a burst of T(4)rII progeny than with either treatment alone. It is suggested that factors produced by both the prophage and the bacterium contribute to the structure or substance responsible for interference. The variation in heat sensitivity of interference in various strains is probably due to differences in the bacterial contribution to the postulated structure.
卡萨蒂亚，尚蒂 - 斯瓦鲁普（南加州大学，洛杉矶）和玛格丽特·利布。热休克对大肠杆菌中T（4）rII增殖的影响。《细菌学杂志》88:1585 - 1589。1964年。 - 在噬菌体感染前将某些大肠杆菌K - 12（λ）菌株加热至45至48摄氏度12分钟，增加了允许T（4）rII突变体增殖的细菌比例。热对T（4）rII干扰的影响与λ原噬菌体的诱导无关，λ原噬菌体诱导在一些研究的菌株中发生。在感染前将加热的细胞在37摄氏度下培养导致干扰恢复，前提是原噬菌体仍然存在。已知感染后镁离子的存在会大大降低K - 12（λ）对T（4）rII的干扰。当用T（4）rII感染热休克细胞，然后在含有次优浓度镁离子的培养基中培养时，与单独的任何一种处理相比，更多的细胞产生了T（4）rII子代的爆发。有人提出，原噬菌体和细菌产生的因子都有助于形成负责干扰的结构或物质。不同菌株中干扰热敏感性的差异可能是由于细菌对假定结构的贡献不同。
