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致敏豚鼠外周血和淋巴结淋巴细胞的体外抗原刺激；体内再次给予抗原的影响。

In vitro antigenic stimulation of peripheral blood and lymph node lymphocytes of sensitized guinea-pigs; the effect of a second administration of antigen in vivo.

作者信息

Housley J, Gell P G

出版信息

Immunology. 1969 Oct;17(4):571-8.

PMID:5352364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1455857/
Abstract

Stimulation of DNA synthesis by a guinea-pig albumin—orthanilic acid conjugate (AO) and by tuberculin purified protein derivative (PPD) was obtained in cultures of peripheral blood and lymph node lymphocytes from guinea-pigs with delayed hypersensitivity to these antigens. Animals sensitized to both AO and PPD were given a further injection of 5 μg AO, intravenously, 8 hours before killing for studies. In these guinea-pigs, peripheral blood cultures, but not lymph node cultures, showed greater DNA synthesis in response to both AO and PPD than cultures from controls not given a further injection of AO. It is suggested that the further increase in DNA synthesis was due to non-specific lymphocyte `activation' following the interaction of antigen and specifically sensitized lymphocytes.

摘要

豚鼠白蛋白 - 邻氨基苯甲酸共轭物（AO）和结核菌素纯化蛋白衍生物（PPD）可刺激对这些抗原有迟发型超敏反应的豚鼠外周血和淋巴结淋巴细胞培养物中的DNA合成。对AO和PPD均致敏的动物在处死进行研究前8小时静脉内再注射5μg AO。在这些豚鼠中，外周血培养物而非淋巴结培养物显示，与未再次注射AO的对照培养物相比，对AO和PPD的反应均有更强的DNA合成。提示DNA合成的进一步增加是由于抗原与特异性致敏淋巴细胞相互作用后非特异性淋巴细胞“活化”所致。

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本文引用的文献

1
ELECTRON MICROSCOPIC OBSERVATIONS ON THE LOCALIZATION OF ANTIGEN IN THE TUBERCULIN REACTION.结核菌素反应中抗原定位的电子显微镜观察
Immunology. 1965 Jan;8(1):81-7.
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ANTIGEN STIMULATED PROLIFERATION IN LYMPHOID AND MYELOID TISSUES FROM IMMUNIZED RABBITS.免疫兔的淋巴组织和骨髓组织中抗原刺激的增殖
Proc Soc Exp Biol Med. 1964 Dec;117:708-11. doi: 10.3181/00379727-117-29674.
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FACTORS AFFECTING THE DYE EXCLUSION TEST FOR CELL VIABILITY.影响细胞活力染料排除试验的因素
Exp Cell Res. 1964 Jun;35:9-13. doi: 10.1016/0014-4827(64)90066-7.
4
Congenital hemihypertrophy and malignancy.先天性半身肥大与恶性肿瘤。
Lancet. 1963 Mar 2;1(7279):468-9. doi: 10.1016/s0140-6736(63)92360-2.
5
Delayed hypersensitivity to hapten-protein conjugates. I. The effect of carrier protein and site of attachment to hapten.对半抗原 - 蛋白质结合物的迟发型超敏反应。I. 载体蛋白及与半抗原连接位点的影响
J Exp Med. 1962 May 1;115(5):1037-51. doi: 10.1084/jem.115.5.1037.
6
Delayed hypersensitivity. III. Specific desensitization of guinea pigs sensitized to protein antigens.迟发型超敏反应。III. 对蛋白质抗原有致敏反应的豚鼠的特异性脱敏
J Exp Med. 1958 Dec 1;108(6):891-904. doi: 10.1084/jem.108.6.891.
7
The activation and reactivation of peripheral lymphocytes in culture.培养中外周淋巴细胞的激活与再激活。
J Cell Sci. 1967 Mar;2(1):57-70. doi: 10.1242/jcs.2.1.57.
8
Delayed hypersensitivity in the guinea-pig to a protein-hapten conjugate and its relationship to in vitro transformation of lymph node, spleen, thymus and peripheral blood lymphocytes.豚鼠对蛋白质-半抗原结合物的迟发型超敏反应及其与淋巴结、脾脏、胸腺和外周血淋巴细胞体外转化的关系。
Immunology. 1967 Jan;12(1):89-102.
9
The immunological significance in the guinea-pig of in vitro transformation of lymph node, spleen and peripheral blood lymphocytes.豚鼠淋巴结、脾脏和外周血淋巴细胞体外转化的免疫学意义。
Immunology. 1968 Feb;14(2):257-64.
10
Immunology of the mycoses. I. Depressed lymphocyte transformation in chronic histoplasmosis.真菌病的免疫学。I. 慢性组织胞浆菌病中淋巴细胞转化受抑制
J Immunol. 1968 Feb;100(2):436-43.