Steele G C, Trinci A P
Arch Microbiol. 1977 May 13;113(1-2):43-8. doi: 10.1007/BF00428578.
Growth of a temperature sensitive colonial mutant (cot 1) of Neurospora crassa was compared with a wild type strain. The hyphal growth unit (the ratio between mycelial length and number of branches) of the wild type was not appreciably altered by temperature and there was a direct relationship between the specific growth rate (alpha) of these mycelia and their mean hyphal extension rate (E). The specific growth rate of cot 1 increased by about the same relative amount as the wild type between 15 degrees and 30 degrees C. Cot 1 grew and branched normally at 15 degrees and 25 degrees C but at 30 degrees C the hyphal growth unit and mean hyphal extension rate of the mutant mycelia were reduced. Thus, between 15-30 degrees C the ratio, E/alpha was constant for the wild type but not for cot 1. The effect of temperature and temperature shifts on extension zone length (2), extension zone expansion time (2t) and branching of leading hyphae of mature colonies were also studied. It is suggested that branching is governed by a mechanism which regulates the linear growth rate of hyphae; the cot 1 mutation may have a direct effect on wall extension or affect linear growth rate indirectly due to an influence on the transport of precursors to the tip.
将粗糙脉孢菌的温度敏感型菌落突变体（cot 1）的生长与野生型菌株进行了比较。野生型的菌丝生长单位（菌丝长度与分支数之比）不受温度的明显影响，并且这些菌丝体的比生长速率（α）与其平均菌丝延伸速率（E）之间存在直接关系。在15摄氏度至30摄氏度之间，cot 1的比生长速率与野生型的增加幅度大致相同。cot 1在15摄氏度和25摄氏度时正常生长和分支，但在30摄氏度时，突变体菌丝体的菌丝生长单位和平均菌丝延伸速率降低。因此，在15至30摄氏度之间，野生型的E/α比值恒定，而cot 1则不然。还研究了温度和温度变化对成熟菌落的延伸区长度（2）、延伸区扩展时间（2t）和领先菌丝分支的影响。有人提出，分支受一种调节菌丝线性生长速率的机制控制；cot 1突变可能对细胞壁延伸有直接影响，或者由于对前体向顶端运输的影响而间接影响线性生长速率。
