HANNA E E, WATSON D W
J Bacteriol. 1965 Jan;89(1):154-8. doi: 10.1128/jb.89.1.154-158.1965.
Hanna, Edgar E. (University of Minnesota, Minneapolis), and Dennis W. Watson. Host-parasite relationships among group A streptococci. III. Depression of reticuloendothelial function by streptococcal pyrogenic exotoxins. J. Bacteriol. 89:154-158. 1965.-Measurement of the rate of clearance of colloidal carbon from the circulating blood is an effective and simple technique for assessing the functional state of the reticuloendothelial system (RES) of experimental animals. This paper concerns a study of the RES function in rabbits after treatment with streptococcal pyrogenic exotoxins. Intravenous injection of 1.0 ml of the toxin produced by a strain of type 18, group A streptococcus caused a prolonged (at least 24 hr) depression of RES function. The activity at 48 hr after toxin injection was about equal to that in control animals. Repeated intravenous injections of the toxin abolished the initial depressing effect on RES function, but did not cause stimulation. In comparison, intravenous injection of equivalent doses of endotoxin prepared from Escherichia coli COO8 also caused an initial depression of RES function; however, in contrast with the effect of exotoxin, stimulation occurred as early as 24 hr and was about twice as active at 48 hr. The prolonged depression by streptococcal pyrogenic exotoxin is suggested as one means by which this toxin potentiates lethality and tissue damage by other toxins such as streptolysin O and endotoxins from gram-negative bacteria.
Hanna, Edgar E.（明尼苏达大学，明尼阿波利斯）以及Dennis W. Watson。A群链球菌的宿主 - 寄生虫关系。III. 链球菌致热外毒素对网状内皮系统功能的抑制。《细菌学杂志》89:154 - 158。1965年。——测量循环血液中胶体碳的清除率是评估实验动物网状内皮系统（RES）功能状态的一种有效且简单的技术。本文涉及对用链球菌致热外毒素处理后的兔子的RES功能的研究。静脉注射1.0毫升由18型A群链球菌菌株产生的毒素会导致RES功能长期（至少24小时）抑制。毒素注射后48小时的活性与对照动物的活性大致相当。重复静脉注射该毒素消除了对RES功能的初始抑制作用，但未引起刺激。相比之下，静脉注射等量的由大肠杆菌COO8制备的内毒素也会导致RES功能的初始抑制；然而，与外毒素的作用不同，早在24小时就出现了刺激，并且在48小时时活性约为两倍。链球菌致热外毒素的长期抑制被认为是该毒素增强其他毒素（如链球菌溶血素O和革兰氏阴性菌内毒素）的致死性和组织损伤的一种方式。
