Yu C E, Ferretti J J
Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City 73190.
Infect Immun. 1989 Dec;57(12):3715-9. doi: 10.1128/iai.57.12.3715-3719.1989.
A molecular epidemiology analysis was performed with over 440 clinical isolates of Streptococcus pyogenes obtained from 11 different countries in order to determine the frequency of occurrence of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) among group A strains. The colony hybridization technique employing a specific internal fragment of the speA gene was used for initial screening, and all positive results were further confirmed by the Southern hybridization technique. Among over 300 general strains obtained from patients with a variety of diseases, except scarlet fever (such as tonsillitis, impetigo, cellulitis, pyoderma, abscess, rheumatic fever, and glomerulonephritis), 15% were found to contain the speA gene. Among a group of 146 strains obtained from individuals described as having scarlet fever, 45% were shown to contain the speA gene. Further analysis of the data indicated that strains with certain M- or T-type surface antigens showed a higher (such as M and T types 1 and 3/13) or lower (such as M2, M12, T4, T5, and T28) tendency to contain the speA gene. No correlation was found between speA content of a strain and the ability to cause a specific disease, although strains possessing the speA gene were more likely to be associated with scarlet fever and rheumatic fever than with other types of disease.
为了确定A群链球菌外毒素（致热毒素）基因（speA）在A群菌株中的出现频率，对从11个不同国家获得的440多株化脓性链球菌临床分离株进行了分子流行病学分析。采用speA基因特异性内部片段的菌落杂交技术进行初步筛选，所有阳性结果均通过Southern杂交技术进一步确认。在从患有各种疾病（如扁桃体炎、脓疱病、蜂窝织炎、脓皮病、脓肿、风湿热和肾小球肾炎）的患者中获得的300多株普通菌株中，发现15%含有speA基因。在从被描述为患有猩红热的个体中获得的一组146株菌株中，45%被证明含有speA基因。对数据的进一步分析表明，具有某些M型或T型表面抗原的菌株含有speA基因的倾向较高（如M型和T型1及3/13）或较低（如M2、M12、T4、T5和T28）。尽管拥有speA基因的菌株比其他类型的疾病更有可能与猩红热和风湿热相关，但未发现菌株的speA含量与引起特定疾病的能力之间存在相关性。
