Cunningham C M, Barsumian E L, Watson D W
Infect Immun. 1976 Sep;14(3):767-75. doi: 10.1128/iai.14.3.767-775.1976.
Streptococcal pyrogenic exotoxin (SPE) isolated from culture filtrates of strain NY-5 (type 10), and separated from other extracellular by differential solubility in ethanol and acetate-buffered saline, has previously been shown to exhibit a wide range of biological activities including erythrogenic activity, pyrogenicity, enhancement of susceptibility to endotoxin shock, blockage of the reticuloendothelial system immmunosuppression, and lymphocyte mitogenicity. Toxin prepared in this way was found to consist of hyaluronic acid and several proteins which could be distinguished by thin-layer polyacrylamide isoelectric focusing (IEF), SPE has been further purified by ion exchange chromatography on QAE-Sephadex columns. One of the fractions isolated from QAE-Sephadex, and shown to be a homogenous protein by thin-layer IEF and Ouchterlony with hyperimmune serum, was highly active erythrogenically, pyrogenically, and in enhancing susceptibility to endotoxin. This fraction was identified as exotoxin A. A second, less active fraction identified as SPE B showed similar activities, but differed from the other fraction antigenically and in net charge and molecular weight. These findings indicate that a single highly purified protein can mediate at least three of the biological activities attributed to SPE and NY-5 produces pyrogenic exotoxins A and B in vitro as well as in vivo.
从菌株NY - 5（10型）培养滤液中分离得到的链球菌致热外毒素（SPE），通过在乙醇和醋酸盐缓冲盐水中的不同溶解度与其他细胞外物质分离，此前已证明其具有广泛的生物学活性，包括致红斑活性、致热性、增强对内毒素休克的易感性、阻断网状内皮系统免疫抑制以及淋巴细胞促有丝分裂活性。发现以这种方式制备的毒素由透明质酸和几种蛋白质组成，这些蛋白质可以通过薄层聚丙烯酰胺等电聚焦（IEF）加以区分，SPE已通过在QAE - 葡聚糖柱上进行离子交换色谱进一步纯化。从QAE - 葡聚糖分离得到的一个组分，通过薄层IEF和与超免疫血清的双向免疫扩散显示为一种纯一蛋白质，具有高度的致红斑活性、致热活性以及增强对内毒素的易感性。该组分被鉴定为外毒素A。第二个活性较低的组分被鉴定为SPE B，显示出类似的活性，但在抗原性、净电荷和分子量方面与另一个组分不同。这些发现表明，一种高度纯化的单一蛋白质可以介导至少三种归因于SPE的生物学活性，并且NY - 5在体外和体内均产生致热外毒素A和B。
