铜绿假单胞菌的核酸外切酶活性在表型无限制突变体中缺失。
Exonuclease activity from Pseudomonas aeruginosa which is missing in phenotypically restrictionless mutants.
作者信息
Potter A A, Loutit J S
出版信息
J Bacteriol. 1982 Sep;151(3):1204-9. doi: 10.1128/jb.151.3.1204-1209.1982.
Abstract
A phenotypically restrictionless strain of Pseudomonas aeruginosa was found to lack a deoxyribonuclease specific for linear duplex DNA. The purified enzyme had an optimum pH of 8.5, required MgCl2 (10 mM) for maximum activity, and did not require ATP. Neither the degradation of heat-denatured DNA nor the degradation of bacteriophage F116 DNA was detected. The genome of bacteriophage F116 was shown to possess single-stranded terminal regions, which account for the resistance to degradation and for the ability of the phage to transfect restriction-proficient strains.
摘要
发现一株表型无限制的铜绿假单胞菌缺乏一种对线性双链DNA具有特异性的脱氧核糖核酸酶。纯化后的酶最适pH值为8.5，最大活性需要MgCl2（10 mM），且不需要ATP。未检测到热变性DNA的降解或噬菌体F116 DNA的降解。噬菌体F116的基因组显示具有单链末端区域，这解释了其对降解的抗性以及噬菌体转染限制能力正常的菌株的能力。
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