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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

J Physiol. 1976 Dec;263(3):475-88. doi: 10.1113/jphysiol.1976.sp011641.

The rate at which CO displaces O2 from its combination with haemoglobin in solution, has been measured spectrophotometrically, using a rapid-mixing stopped-flow technique. 2. In the presence of CO2, the reaction proceeds by a unimolecular dissociation, with a rate constant r. 3. The relationship of the reciprocal of r to the ratio PO2/PCO is nonlinear, and a different curve is obtained at each CO concentration. 4. Measurements were made of the rate of the reaction when the pH was varied, with constant or varying PCO2. In both situations the value of r was found to have a miximum, for a given PO2/PCO ratio and CO cencentration, at pH 7.2. 5. An analysis of these results suggest that the Bohr effect, of pH and PCO2, is a dynamic equilibrium between four stable tertiary states of each of the alpha and beta chains. Each intermediatory complex has different rate constants for CO and O2.

采用快速混合停流技术，通过分光光度法测量了一氧化碳（CO）从溶液中与血红蛋白结合的氧（O₂）中置换出来的速率。2. 在二氧化碳（CO₂）存在的情况下，反应通过单分子解离进行，速率常数为r。3. r的倒数与氧分压（PO₂）/一氧化碳分压（PCO）之比的关系是非线性的，并且在每个CO浓度下都会得到不同的曲线。4. 在二氧化碳分压恒定或变化的情况下，当pH值变化时测量了反应速率。在这两种情况下，对于给定的PO₂/PCO比值和CO浓度，发现在pH 7.2时r值有一个最大值。5. 对这些结果的分析表明，pH值和PCO₂的波尔效应是α链和β链各自四个稳定三级状态之间的动态平衡。每个中间复合物对CO和O₂具有不同的速率常数。

J Physiol. 1976 Dec;263(3):475-88. doi: 10.1113/jphysiol.1976.sp011641.

The rate at which CO displaces O2 from its combination with haemoglobin in solution, has been measured spectrophotometrically, using a rapid-mixing stopped-flow technique. 2. In the presence of CO2, the reaction proceeds by a unimolecular dissociation, with a rate constant r. 3. The relationship of the reciprocal of r to the ratio PO2/PCO is nonlinear, and a different curve is obtained at each CO concentration. 4. Measurements were made of the rate of the reaction when the pH was varied, with constant or varying PCO2. In both situations the value of r was found to have a miximum, for a given PO2/PCO ratio and CO cencentration, at pH 7.2. 5. An analysis of these results suggest that the Bohr effect, of pH and PCO2, is a dynamic equilibrium between four stable tertiary states of each of the alpha and beta chains. Each intermediatory complex has different rate constants for CO and O2.

采用快速混合停流技术，通过分光光度法测量了一氧化碳（CO）从溶液中与血红蛋白结合的氧（O₂）中置换出来的速率。2. 在二氧化碳（CO₂）存在的情况下，反应通过单分子解离进行，速率常数为r。3. r的倒数与氧分压（PO₂）/一氧化碳分压（PCO）之比的关系是非线性的，并且在每个CO浓度下都会得到不同的曲线。4. 在二氧化碳分压恒定或变化的情况下，当pH值变化时测量了反应速率。在这两种情况下，对于给定的PO₂/PCO比值和CO浓度，发现在pH 7.2时r值有一个最大值。5. 对这些结果的分析表明，pH值和PCO₂的波尔效应是α链和β链各自四个稳定三级状态之间的动态平衡。每个中间复合物对CO和O₂具有不同的速率常数。