Barak V, Yanai P, Treves A J, Roisman I, Simon A, Laufer N
Department of Oncology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Fertil Steril. 1992 Oct;58(4):719-25. doi: 10.1016/s0015-0282(16)55318-7.
To investigate the possibility of local interleukin-1 (IL-1) and IL-1 inhibitor production by human granulosa and cumulus cells and to assess their direct effects on the steroidogenesis of these cells in vitro.
Prospective study.
Normal ovulatory women undergoing ovulation induction for in vitro fertilization.
Pretreatment of patients with gonadotropin-releasing hormone analogue, human menopausal gonadotropin, and human chorionic gonadotropin (hCG).
Retrieval and isolation of granulosa luteal cells and follicular fluid (FF). Granulosa luteal cells and cumulus cells cultured and analyzed by fluorescent activated cell sorter. Follicular fluid separated and bioassayed for IL-1 and IL-1 inhibitory activity. Steroid measurement performed. Interleukin-1 inhibitor purified. Interleukin-1 and IL-1 inhibitor bioassay performed. Statistical analysis made and interpreted.
Interleukin-1, but not IL-1 specific inhibitory activity, was found in granulosa and cumulus cell cultures and also in FF, only after its purification on a high-pressure liquid chromatography column. Under nonstimulated conditions, neither IL-1 nor IL-1 inhibitor had any effect on basal progesterone (P) or estradiol (E2) secretion. However, IL-1 inhibitor demonstrated significant (P < 0.01) inhibition of hCG-stimulated P secretion (from 200 to 110 ng/10,000 cells per 24 hours). In addition, IL-1 demonstrated a significant (P < 0.05) and dose-dependent inhibition of hCG-stimulated E2 production (from 6,832 +/- 460 to 4,237 +/- 141 pg/10,000 cells per 24 hours).
Interleukin-1 may exert a significant local autocrine regulatory role in the human ovary.
研究人颗粒细胞和卵丘细胞产生局部白细胞介素-1（IL-1）及IL-1抑制剂的可能性，并评估它们在体外对这些细胞类固醇生成的直接影响。
前瞻性研究。
接受体外受精排卵诱导的正常排卵女性。
用促性腺激素释放激素类似物、人绝经期促性腺激素和人绒毛膜促性腺激素（hCG）对患者进行预处理。
黄体颗粒细胞和卵泡液（FF）的获取与分离。黄体颗粒细胞和卵丘细胞经荧光激活细胞分选仪培养和分析。卵泡液分离并进行IL-1和IL-1抑制活性的生物测定。进行类固醇测量。纯化白细胞介素-1抑制剂。进行白细胞介素-1和IL-1抑制剂生物测定。进行统计分析并解释结果。
仅在高压液相色谱柱上纯化后，才在颗粒细胞和卵丘细胞培养物以及卵泡液中发现白细胞介素-1，而未发现IL-1特异性抑制活性。在非刺激条件下，IL-1和IL-1抑制剂对基础孕酮（P）或雌二醇（E2）分泌均无任何影响。然而，IL-1抑制剂对hCG刺激的P分泌表现出显著（P<0.01）抑制作用（从每24小时每10,000个细胞200纳克降至110纳克）。此外，IL-1对hCG刺激的E2产生表现出显著（P<0.05）且剂量依赖性的抑制作用（从每24小时每10,000个细胞6,832±460皮克降至4,237±141皮克）。
白细胞介素-1可能在人类卵巢中发挥重要的局部自分泌调节作用。
